Test: GRE Subject Test: Biology

Bacterial transformations are a useful tool for biologists to replicate valuable plasmid DNA so that it can be used for a myriad of experiments. A student researcher has designed an experiment to determine the effects of pre-incubation duration, heat shock duration, and heat shock temperature on transformation efficiency. The student transformed 1μg of a β-lactamase encoding plasmid and recovered the bacteria for 1 hour at 37°C (shaking at 225rpm) for all conditions in Table 1. He then plated the transformed bacteria on ampicillin agar places and observed growth 24 hours later. Table 1 outlines the variables in the experiment. 

 

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1.

Of the conditions tested, which condition is optimal to generate a high transformation yield?

Pre-incubation 60min, heat shock duration 60sec, heat shock temp 42°C

Pre-incubation 30min, heat shock duration 60sec, heat shock temp 42°C

Pre-incubation 30min, heat shock duration 60sec, heat shock temp 39°C

Pre-incubation 30min, heat shock duration 30sec, heat shock temp 42°C

Pre-incubation 60min, heat shock duration 30sec, heat shock temp 42°C

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