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Test: GRE Subject Test: Biology
Bacterial transformations are a useful tool for biologists to replicate valuable plasmid DNA so that it can be used for a myriad of experiments. A student researcher has designed an experiment to determine the effects of pre-incubation duration, heat shock duration, and heat shock temperature on transformation efficiency. The student transformed 1μg of a β-lactamase encoding plasmid and recovered the bacteria for 1 hour at 37°C (shaking at 225rpm) for all conditions in Table 1. He then plated the transformed bacteria on ampicillin agar places and observed growth 24 hours later. Table 1 outlines the variables in the experiment.
| 1. | Besides the indicated variables in table 1, what is most likely the next variable the student would introduce in this experiment to optimize transformation efficiency? |
Chemical competent bacteria verses electro-competent bacteria
None of the other answers
Varying amounts of plasmid DNA
Water incubators verses air-vented incubators
Varying concentrations of ampicillin
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