Test: GRE Subject Test: Biochemistry, Cell, and Molecular Biology

1.

When performing whole-mount immunofluorescence of a specimen, background staining is often a concern with custom generated antibodies. Which of the following is not a way in which to increase specificity of the custom antibody? 

Generate the custom antibody against purified protein rather than a predictive peptide

Affinity purify the custom antisera

Determine optimal blocking buffer components and concentrations 

None of the other answers 

Determine optimal antibody dilution for the application by performing serial dilutions 

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