Test: GRE Subject Test: Biochemistry, Cell, and Molecular Biology

1.

When performing whole-mount immunofluorescence of a specimen, background staining is often a concern with custom generated antibodies. Which of the following is not a way in which to increase specificity of the custom antibody? 

Determine optimal blocking buffer components and concentrations 

None of the other answers 

Generate the custom antibody against purified protein rather than a predictive peptide

Affinity purify the custom antisera

Determine optimal antibody dilution for the application by performing serial dilutions 

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