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Test: GRE Subject Test: Biochemistry, Cell, and Molecular Biology
Two students have cloned a bacterial DNA polymerase into a mammalian expression vector. The students then transfected the vector into human tissue culture cell lines to determine if the DNA polymerase retains its native function in human cells.
1. | What technique is most suitable for the students to use to determine if the DNA polymerase is expressed in human cells? |
Polymerase chain reaction (PCR)
Electrophoretic mobility shift assay (EMSA)
Restriction digestion
Western blot
Electroporation
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