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MCAT Chemical and Physical Foundations of Biological Systems Flashcards: 5c Electrophoresis Protein Separation

Study 5c Electrophoresis Protein Separation in MCAT Chemical and Physical Foundations of Biological Systems with focused flashcards that help you recognize the idea, recall the key rule, and apply it in practice-style prompts.

QUESTION

What is the main role of SDS in SDS-PAGE sample preparation?

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ANSWER

Denatures proteins and imparts a uniform negative charge per mass. As an anionic detergent, SDS unfolds proteins and binds proportionally to length, providing consistent negative charge.

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MCAT Chemical and Physical Foundations of Biological Systems: Foundational Concepts

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This deck focuses on 5c Electrophoresis Protein Separation, giving you a quick way to review the definitions, rules, and examples that matter most for MCAT Chemical and Physical Foundations of Biological Systems.

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Work through these flashcards in short sessions. Try to answer each prompt before flipping the card, then revisit any cards you miss until the explanation feels automatic.

Practice questions

1 of 50Practice questions for this set
A researcher performed SDS-PAGE (pH 8.3) on two proteins D1 and D2 that differ in MW (D1: 15 kDa; D2: 150 kDa). At 120 V for 50 min, D1 migrated 6.5 cm and D2 migrated 1.8 cm. The researcher then doubled the voltage but kept run time the same; both proteins migrated farther, but the distance between their bands (in cm) decreased. Which factor most likely contributed to the decreased band spacing at higher voltage?
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