Classifications of Enzymes - MCAT Chemical and Physical Foundations of Biological Systems

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Question

Chymotrypsin is a proteolytic enzyme that is released by the pancreas. Using a water molecule, it cleaves the traget polypeptide and creates the new N and C termini for the newly made fragments.

Based on this enzymatic function, what type of enzyme is chymotrypsin?

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Answer

Chymostrypsin creates smaller peptide segments, so it is not a ligase or transferase. Since it employs the use of a water molecule in order to cleave the polymer, it is classified as a hydrolase enzyme. Lyases also shorten polymers, but not with the use of a water molecule.

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