While Christian iconography and themes feature as the most common subjects in Western art, certain periods were more heavily indebted to Christian tradition. During the period of Gothic art, which was roughly synonymous with the twelfth, thirteenth, and fourteenth centuries in Europe, Christian themes were almost the only ones used. Even in royal portraits, such as the Wilton Diptych from England, featured saints and angels next to the King.

Saint Stephen is traditionally considered the first martyr of Christianity, and his death by stoning is told in the biblical book of Acts. Due to the circumstances of his death, he was usually depicted with a stone implanted in his skull. Thanks to his position as "the first martyr," Saint Stephen very commonly depicted in Medieval art.

This painting is by the Medieval artist Fra Angelico and known as the Cortona Annunciation. Painted from 1433-1434, it depicts the archangel Gabriel telling the Virgin Mary that she is pregnant with the Christ child. The Annunciation, as a key moment in the Bible, was a very popular theme in Medieval European art. This particular painting was part of an altarpiece in a church in Cortona, Italy, and painted by a priest.

Saint Sebastian was a martyr of the early Christian church who died in the Diocletian persecutions of the third century CE. He became a popular figure in medieval art, usually shown as being tied to a tree with arrows in his sides. This image was to convey what an appropriate Christian martyr should look like.

As one of the most revered figures in Christianity, the Virgin Mary was a constant theme of medieval religious art. In order to function as a highly visible symbol in a mostly illiterate culture, many different symbols were used to distinguish Mary. Most important was her robe, which was typically a bright blue color, which denoted expensive silks.