AP Biology › Understanding the Citric Acid Cycle
A sample of rats were fed glucose containing radioactive oxygen. After a few minutes, where would the radioactive oxygen be found?
In cellular respiration, glucose first undergoes glycolysis and is broken down into two pyruvate molecules. As the pyruvate passes through the citric acid cycle, three molecules of are produced. The radioactive oxygen molecules would be found in the
.
is formed when electrons removed from glucose are used to reduce
.
is produced by the phosphorylation of
. The oxygen in
enters the mitochondrion as gaseous molecular oxygen from the atmosphere, not from glucose. Finally,
is reduced to water in cellular respiration and serves as a reactant, rather than a product, in cell metabolism.
Under aerobic conditions, pyruvate produced from glycolysis is metabolized through the citric acid cycle.
The products of the citric acid cycle include all of the following except __________.
Upon completion of the citric acid cycle, 1 molecule of , 2 molecules of
, 3 molecules of
, and 1 molecule of
are produced.
is not produced during the citric acid cycle.
is the product of the pentose phosphate pathway.
is a powerful reducing agent used in several metabolic pathways. For example, it is used in red blood cells to reduce glutathione. Note that the products listed above represent those from one turn of the citric acid cycle; each molecule of glucose produces two molecules of acetyl-CoA, thus the cycle turns twice per glucose molecule.
For each molecule of glucose metabolized, the citric acid cycle produces __________.
six NADH molecules and two FADH2 molecules
thirty-two ATP molecules
an electrochemical gradient
three NADH molecules and one FADH2 molecule
one NAD+ molecule
Glycolysis generates four ATP per molecule of glucose and uses up two of those in the process, while oxidative phosphorylation produces thirty-two ATP per molecule of glucose. Fermentation produces NAD+, which is used in glycolysis. The citric acid cycle produces NADH and FADH2, which are used in the electric transport chain. Note that for each turn of the citric acid cycle, three NADH molecules and one FADH2 molecule is produced, but each glucose produces two pyruvate molecules, which are converted to two acetyl-CoA molecules. Each acetyl-CoA molecule results in one full turn of the citric acid cycle. The electron transport chain produces an electrochemical gradient across the inner membrane of the mitochondria, which provides the energy used by oxidative phosphorylation to produce thirty-two ATP per molecule of glucose.
Where does the citric acid (Krebs) cycle occur in human cells?
Mitochondrial matrix
Ribosomes
Inner mitochondrial membrane
Nucleus
Cytoplasm
The citric acid (Krebs) cycle takes place in the mitochondrial matrix. The Krebs cycle involves using acetyl-CoA as a substrate to produce high energy electron carriers and
to later participate in electron transport, ultimately yielding
. Glycolysis is the process by which glucose is split into two molecules of pyruvate, and occurs in the cytoplasm. The electron transport chain performs its function in the inner mitochondrial membrane. The nucleus and ribosomes are not part of the citric acid cycle.
In the Krebs cycle, what compound is formed when two carbon atoms from pyruvic acid are joined to coenzyme A?
Acetyl-CoA
Ribose
ATP
FAD
Acetyl-CoA is formed when two carbon atoms join to coenzyme A. Ribose is a five carbon sugar in RNA. ATP is an energy compound, and FAD is an electron carrier in the Krebs cycle which gets reduced then passes its electron to an enzyme complex in the electron transport chain.
In the Krebs cycle, what is the gas released as waste?
Carbon dioxide
Oxygen
Nitrogen
Carbon monoxide
Carbon dioxide is the gas produced in the Krebs cycle, which animals exhale. Oxygen is used as an electron acceptor, while nitrogen is not a waste gas. Carbon monoxide is not a waste product in the Krebs cycle.
Which of the following is a correct summation of the products of a single glucose molecule after it has gone through the Krebs cycle?
E.10 NADH, 4 ATP, 2 FADH2
10 NDAH, 4 FADH2, 2 ATP
10 ATP, 4 NADH, 2 FADH2
10 NADH, 4 FADH2, 2 ATP
10 ATP, 4 FADH2, 2 NADH
This is because during glycolysis, a glucose molecule produces 2 ATP, 2 NADH, and 2 pyruvate. The 2 pyruvate created are used again to create 8 NADH, 2 FADH2, and 2 ATP during the Krebs cycle to net 10 NADH, 4 ATP, 2 FADH2.
During lactic acid fermentation, which of these events is least likely to occur?
is reduced
An oxygen debt accumulates
Lactate builds in the muscle tissue
ATP is created
is recycled
The answer to this question is is reduced.
is least likely to occur because it is already in its reduced form. In actuality, it gets oxidized, as pyruvate is reduced into lactic acid. These other events are likely to occur. Your body does lack oxygen and lactate and ATP are produced in order to make up for the debt of oxygen.
also is recycled to be used in glycolysis so this event is also not least likely to occur.
In cellular respiration, in what organelle does the Krebs cycle take place?
Mitochondria
Golgi apparatus
Chloroplast
Nucleus
The Krebs cycle takes place in the mitochondria. The Golgi apparatus packages substances in order for them to be sent to their appropriate locations, and the chloroplast is where photosynthesis takes place. The nucleus contains DNA and is the site of transcription.
What type of respiration is involved in the Krebs cycle?
Aerobic
Anaerobic
Fermentation
Lactic acid
The Krebs cycle involves aerobic respiration because oxygen is used. Anaerobic respiration involves fermentation because oxygen is lacking and the compound does not enter the Krebs cycle. Glycolysis is the first step in respiration, which precedes the Krebs cycle and is fully anaerobic. Note that oxygen is not directly required for the Krebs cycle (it is not a reactant), however, without oxygen, the electron transport chain will not proceed and thus the Krebs cycle will also be halted.