Understanding Punnett Squares and Test Crosses - AP Biology

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Question

Let us assume that flower color is either purple or white in a particular species. Color is determined by complete dominance, and purple is dominant to white.

A purple flower sprouts in the garden one day, and the gardener would like to know if it will only create purple flowers if it is pollinated. If she wants to use only one generation to determine its genotype, how should she pollinate the flower?

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Answer

When attempting to determine if an organism is heterozygous or homozygous for a dominant trait, it is best to use a test cross. A test cross involves crossing the flower in question with a homozygous recessive flower. Since a white flower can only contribute a white allele, we can determine if the purple flower in question is heterozygous or homozygous. Any white flowers in the next generation will confirm that the purple flower is heterozygous. If they are all purple, we can confirm that the flower is homozygous for the trait.

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