Experimental Design

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ACT Science › Experimental Design

Questions 1 - 10
1

In Study 1, the animals were kept "at rest." This was an important experimental control because:

measuring oxygen consumption is impossible while animals are moving.

lizards cannot run at temperatures below 10°C.

it ensured that the environmental temperature remained stable.

activity level significantly affects metabolic rate, as shown in Study 2.

Explanation

This is an experimental design question about why a specific control was necessary. Study 1 tested the effect of temperature on metabolic rate. To isolate temperature as the only variable, activity level had to be controlled (kept constant at rest). Study 2 directly demonstrates why this control matters—it shows that running dramatically increases metabolic rate (Mouse: 1.5 → 8.5; Lizard: 0.5 → 3.2). If animals had been active in Study 1, you couldn't distinguish whether metabolic changes were due to temperature or activity. Choice B correctly identifies this control's purpose and cites the supporting evidence. Choice A is backwards—keeping animals still doesn't control temperature. Choice C is unsupported speculation. Choice D is factually wrong—measurement is possible during activity (that's what Study 2 did). Pro tip: Controls eliminate confounding variables—look for evidence elsewhere in the passage that shows why that variable needed controlling.

2

In Study 3, the students included Trial 6 (0.0% NaHCO₃) primarily to:

serve as a control to verify that CO₂ is necessary for photosynthesis.

determine the maximum possible rate of photosynthesis.

measure the amount of oxygen dissolved in the water before the experiment began.

prove that Elodea can produce oxygen without carbon dioxide.

Explanation

This is an experimental design question about controls. Trial 6 with 0.0% NaHCO₃ represents the absence of the carbon dioxide source. By including this negative control (no CO₂), students can verify that CO₂ is actually necessary—if bubbles still appear without CO₂, it would invalidate their assumption. The fact that only 2 bubbles appeared (compared to 45+ with CO₂) confirms CO₂ is essential. Choice B correctly identifies this control purpose. Choice A (maximum rate) would require optimal conditions, not zero CO₂. Choice C (produce without CO₂) contradicts the data (only 2 bubbles suggests minimal activity). Choice D (pre-existing oxygen) isn't the control's purpose. Pro tip: Trials with zero concentration of the independent variable are negative controls testing necessity.

3

In Study 1, the independent variable (the factor intentionally changed by the student) was:

the mass of the block.

the angle of the incline.

the force measured by the spring scale.

the surface material.

Explanation

This is an experimental design question about variables. The independent variable is what the experimenter deliberately manipulates to see its effect. Study 1's procedure states: "The student varied the angle of the incline from 10° to 60°." This shows the angle was intentionally changed across trials. Choice B is correct. Choice A (mass) was kept constant at 1.0 kg in Study 1. Choice C (force) is the dependent variable being measured, not manipulated. Choice D (surface material) was kept constant (smooth wood) in Study 1. Pro tip: Independent variables are described with words like "varied," "changed," or "tested." Dependent variables are "measured" or "recorded."

4

In an experiment, researchers wanted to determine the effect of sunlight on plant growth. They placed 20 plants in a greenhouse, with 10 plants receiving 6 hours of sunlight per day and the other 10 receiving no sunlight. At the end of four weeks, they measured the height of each plant. What is the independent variable in this experiment?

Amount of sunlight received

Duration of the experiment

Type of plant used

Height of the plants

Explanation

The experiment tests whether sunlight exposure affects plant growth by comparing plants receiving different amounts of sunlight. The researchers manipulate the amount of sunlight (6 hours vs. no sunlight) while keeping other factors constant, allowing them to isolate sunlight's effect on growth. The independent variable is the amount of sunlight received because this is the factor being deliberately varied by the researchers. Duration and plant type are held constant, while height is the measured outcome (dependent variable).

5

Scientists are testing whether a new fertilizer increases crop yield compared to no fertilizer. A flaw in this experimental design is that:

There is no measurement of yield.

There is no randomization of plots.

The control group is unaccounted for.

Fertilizer amount is not varied.

Explanation

The hypothesis is that the new fertilizer increases crop yield compared to no fertilizer treatment. A critical flaw in this experimental design is the lack of randomization of plots, which could lead to bias if certain plots have inherently better or worse growing conditions. Without randomizing which plots receive fertilizer versus control treatment, any differences in yield might be due to plot location, soil quality, or other environmental factors rather than the fertilizer itself. Randomization is essential to ensure that treatment and control groups are comparable.

6

In an experiment to measure the effect of temperature on the solubility of a salt, a student dissolves the same amount of salt in 100 mL of water at 5°C, 25°C, and 50°C. The primary purpose of this experiment was to:

Examine the effect of salt concentration on water temperature.

Test the effect of water volume on temperature.

Determine how temperature affects salt solubility.

Find the best temperature for salt preservation.

Explanation

The hypothesis is that temperature affects the solubility of salt in water. The experiment systematically varies temperature (5°C, 25°C, and 50°C) while keeping other factors constant (same amount of salt, same volume of water), allowing the effect of temperature on solubility to be isolated and measured. The primary purpose is to determine how temperature affects salt solubility, which directly matches the experimental design of testing dissolution at different temperatures. Option A correctly captures this relationship between the independent variable (temperature) and dependent variable (solubility).

7

In a study testing whether temperature affects bacterial growth, cultures are grown at varying temperatures. Which hypothesis is being tested?

Nutrient variation affects growth.

Temperature influences bacterial growth.

Bacterial growth is independent of temperature.

Temperature affects bacterial color.

Explanation

The hypothesis being tested is that temperature influences bacterial growth rates. The experimental design grows bacterial cultures at varying temperatures to test whether temperature changes affect how rapidly bacteria reproduce and multiply. This systematic variation of temperature allows researchers to determine if thermal conditions are a significant factor in bacterial proliferation. Option A correctly identifies that the study examines temperature's influence on bacterial growth rather than other factors like color or nutrient variation.

8

Researchers tested the effect of light intensity on plant growth using three groups of plants exposed to low, medium, and high light. What is the independent variable in this experiment?

Light intensity

Number of plants

Plant growth

Watering frequency

Explanation

The hypothesis being tested is that light intensity affects plant growth rates. The experimental design manipulates light intensity (low, medium, high) as the factor being changed by the researchers, making it the independent variable - the variable that is deliberately controlled and varied to test its effect. Plant growth would be the dependent variable (what is measured), while factors like number of plants and watering frequency would be controlled variables. The independent variable is always the factor that researchers intentionally manipulate to observe its effects.

9

An experiment tests the effect of water salinity on fish respiration rate by altering salinity levels. What is the independent variable?

Fish respiration rate

Type of fish

Water salinity

Water temperature

Explanation

The hypothesis is that water salinity affects fish respiration rates. The experimental design alters salinity levels in the water to test their impact on fish breathing patterns, making water salinity the independent variable - the factor being systematically manipulated by researchers. Fish respiration rate would be the dependent variable (what's measured), while factors like type of fish and water temperature would ideally be controlled. Salinity serves as the environmental variable being tested for its physiological effects.

10

Researchers are studying the effect of music on concentration. Participants performed a task in silence or with background music, and their performance was measured. The independent variable is:

Presence of background music

Duration of the task

Type of task performed

Participants' task performance

Explanation

The experiment tests whether background music affects concentration by comparing task performance under two conditions: silence versus music. The researchers manipulate the auditory environment (music presence/absence) and measure the resulting performance changes. The independent variable is the presence of background music because this is the factor being deliberately controlled and varied by the researchers. Task performance is the dependent variable (what's measured), while task type and duration are held constant across conditions.

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