Microbiology

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Questions 1 - 10
1

Herpes virus can be detected by the Tzanck smear of the skin lesion. This test stains for the presence of which one of the following?

Multinucleated giant cells

Inclusion bodies

Dead cells

Herpes DNA

Herpes cell envelope

Explanation

The Tzanck smear stains for the presence of multinucleated giant cells that are formed as a result of fusion of cell membranes due to insertion of viral proteins into the membrane. Tzanck smear does not detect the presence of inclusion bodies, dead cells, herpes DNA, or cell envelope.

2

Which of the following is true of human adenoviruses?

Encodes for its own DNA polymerase.

They are enveloped viruses with helical nucleocapsid.

Genome consists of a circular single stranded RNA.

Forms acidophilic intracytoplasmic inclusions.

Replication occurs in the cytoplasm.

Explanation

Adenoviruses are non-enveloped viruses with an icosahedral nucleocapsid. The capsid contains 12 pentons at each vertices with 12 fibers projecting from the vertices. The genome is a linear double stranded DNA with terminal protein. Viral attachment proteins are present in the fibers projecting from the vertices of the virions.

The additional characteristics of adenovirus include the following:

  • The virions replicate in the nucleus
  • Encode proteins to promote mRNA and DNA synthesis, including its own DNA polymerase
  • Infects epithelial cells lining respiratory and enteric organs
  • Virus can become latent in lymphoid tissue and can be reactivated by immunosuppression or infection with other agents
  • Forms basophilic intranuclear inclusions within an infected epithelial cell

Last, they are commonly associated with respiratory diseases, adenoviral pneumonia, epidemic keratoconjunctivitis, and pharyngoconjunctival fever.

3

Which agar would be the best choice option to identify_Escherichia coli_?

EMB (eosin methylene blue), because it is selective and differential

Nutrient agar

Selenium enriched media

Phenethyl alcohol

Explanation

The question requires the knowledge that Escherichia coli is a facultative, Gram negative, lactose fermenting bacteria. EMB (eosin methylene blue), or Mac (MacConkey) media is superior for isolating Escherichia coli because it is both selective and differential. Nutrient agar is neither selective nor differential. Selenium enriched media would not help identify this bacteria because Escherichia coli does not require this supplemented media to grow. Phenethyl alcohol can be used to select for anaerobic bacteria, which is of no utility in this situation.

4

Herpes virus can be detected by the Tzanck smear of the skin lesion. This test stains for the presence of which one of the following?

Multinucleated giant cells

Inclusion bodies

Dead cells

Herpes DNA

Herpes cell envelope

Explanation

The Tzanck smear stains for the presence of multinucleated giant cells that are formed as a result of fusion of cell membranes due to insertion of viral proteins into the membrane. Tzanck smear does not detect the presence of inclusion bodies, dead cells, herpes DNA, or cell envelope.

5

Which of the following is true of human adenoviruses?

Encodes for its own DNA polymerase.

They are enveloped viruses with helical nucleocapsid.

Genome consists of a circular single stranded RNA.

Forms acidophilic intracytoplasmic inclusions.

Replication occurs in the cytoplasm.

Explanation

Adenoviruses are non-enveloped viruses with an icosahedral nucleocapsid. The capsid contains 12 pentons at each vertices with 12 fibers projecting from the vertices. The genome is a linear double stranded DNA with terminal protein. Viral attachment proteins are present in the fibers projecting from the vertices of the virions.

The additional characteristics of adenovirus include the following:

  • The virions replicate in the nucleus
  • Encode proteins to promote mRNA and DNA synthesis, including its own DNA polymerase
  • Infects epithelial cells lining respiratory and enteric organs
  • Virus can become latent in lymphoid tissue and can be reactivated by immunosuppression or infection with other agents
  • Forms basophilic intranuclear inclusions within an infected epithelial cell

Last, they are commonly associated with respiratory diseases, adenoviral pneumonia, epidemic keratoconjunctivitis, and pharyngoconjunctival fever.

6

Which agar would be the best choice option to identify_Escherichia coli_?

EMB (eosin methylene blue), because it is selective and differential

Nutrient agar

Selenium enriched media

Phenethyl alcohol

Explanation

The question requires the knowledge that Escherichia coli is a facultative, Gram negative, lactose fermenting bacteria. EMB (eosin methylene blue), or Mac (MacConkey) media is superior for isolating Escherichia coli because it is both selective and differential. Nutrient agar is neither selective nor differential. Selenium enriched media would not help identify this bacteria because Escherichia coli does not require this supplemented media to grow. Phenethyl alcohol can be used to select for anaerobic bacteria, which is of no utility in this situation.

7

Herpes virus can be detected by the Tzanck smear of the skin lesion. This test stains for the presence of which one of the following?

Multinucleated giant cells

Inclusion bodies

Dead cells

Herpes DNA

Herpes cell envelope

Explanation

The Tzanck smear stains for the presence of multinucleated giant cells that are formed as a result of fusion of cell membranes due to insertion of viral proteins into the membrane. Tzanck smear does not detect the presence of inclusion bodies, dead cells, herpes DNA, or cell envelope.

8

Which of the following is true of human adenoviruses?

Encodes for its own DNA polymerase.

They are enveloped viruses with helical nucleocapsid.

Genome consists of a circular single stranded RNA.

Forms acidophilic intracytoplasmic inclusions.

Replication occurs in the cytoplasm.

Explanation

Adenoviruses are non-enveloped viruses with an icosahedral nucleocapsid. The capsid contains 12 pentons at each vertices with 12 fibers projecting from the vertices. The genome is a linear double stranded DNA with terminal protein. Viral attachment proteins are present in the fibers projecting from the vertices of the virions.

The additional characteristics of adenovirus include the following:

  • The virions replicate in the nucleus
  • Encode proteins to promote mRNA and DNA synthesis, including its own DNA polymerase
  • Infects epithelial cells lining respiratory and enteric organs
  • Virus can become latent in lymphoid tissue and can be reactivated by immunosuppression or infection with other agents
  • Forms basophilic intranuclear inclusions within an infected epithelial cell

Last, they are commonly associated with respiratory diseases, adenoviral pneumonia, epidemic keratoconjunctivitis, and pharyngoconjunctival fever.

9

Which agar would be the best choice option to identify_Escherichia coli_?

EMB (eosin methylene blue), because it is selective and differential

Nutrient agar

Selenium enriched media

Phenethyl alcohol

Explanation

The question requires the knowledge that Escherichia coli is a facultative, Gram negative, lactose fermenting bacteria. EMB (eosin methylene blue), or Mac (MacConkey) media is superior for isolating Escherichia coli because it is both selective and differential. Nutrient agar is neither selective nor differential. Selenium enriched media would not help identify this bacteria because Escherichia coli does not require this supplemented media to grow. Phenethyl alcohol can be used to select for anaerobic bacteria, which is of no utility in this situation.

10

An epidemiologist at a school of public health of a major medical school examined data from several studies performed by microbiologist. The data set included information about the nature of the etiological agent (virus, rickettsia, chlamydia, viroid), the nature of the genome (DNA or RNA), and whether the agent is obligate intracellular or parasitic. The epidemiologist wishes to determine which features pertain to each disease agent. Which of the following properties is unique to viruses alone?

Which of the following properties is unique to viruses alone?

Presence of an RNA genome

Obligate intracellular

Parasitic

Presence of DNA genome

Extracellular

Explanation

Rickettsia, chlamydia, prions, and viroids can be distinguished from viruses, but not by just one of the mentioned criteria. While all of these groups are obligate intracellular organisms, only the viruses are parasites with an absolute intracellular requirement, which also depend on the machinery of the host cell for replication. Prions are small infectious protein particles. Viroids are subviral infectious nucleic acids, which lack even the protein capsid found in viruses. Viruses may contain a genome of DNA or RNA, so the presence of an RNA genome is indicative of a virus, but the presence of a DNA genome is not.

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