Drawing Evidence from Natural Science Passages - SAT Critical Reading

Adapted from "Recent Views as to Direct Action of Light on the Colors of Flowers and Fruits" in Tropical Nature, and Other Essays by Alfred Russel Wallace (1878)

The theory that the brilliant colors of flowers and fruits is due to the direct action of light has been supported by a recent writer by examples taken from the arctic instead of from the tropical flora. In the arctic regions, vegetation is excessively rapid during the short summer, and this is held to be due to the continuous action of light throughout the long summer days. "The further we advance towards the north, the more the leaves of plants increase in size as if to absorb a greater proportion of the solar rays. M. Grisebach says that during a journey in Norway he observed that the majority of deciduous trees had already, at the 60th degree of latitude, larger leaves than in Germany, while M. Ch. Martins has made a similar observation as regards the leguminous plants cultivated in Lapland.” The same writer goes on to say that all the seeds of cultivated plants acquire a deeper color the further north they are grown, white haricots becoming brown or black, and white wheat becoming brown, while the green color of all vegetation becomes more intense. The flowers also are similarly changed: those which are white or yellow in central Europe becoming red or orange in Norway. This is what occurs in the Alpine flora, and the cause is said to be the same in both—the greater intensity of the sunlight. In the one the light is more persistent, in the other more intense because it traverses a less thickness of atmosphere.

Admitting the facts as above stated to be in themselves correct, they do not by any means establish the theory founded on them; and it is curious that Grisebach, who has been quoted by this writer for the fact of the increased size of the foliage, gives a totally different explanation of the more vivid colors of Arctic flowers. He says, “We see flowers become larger and more richly colored in proportion as, by the increasing length of winter, insects become rarer, and their cooperation in the act of fecundation is exposed to more uncertain chances.” (Vegetation du Globe, col. i. p. 61—French translation.) This is the theory here adopted to explain the colors of Alpine plants, and we believe there are many facts that will show it to be the preferable one. The statement that the white and yellow flowers of temperate Europe become red or golden in the Arctic regions must we think be incorrect. By roughly tabulating the colors of the plants given by Sir Joseph Hooker as permanently Arctic, we find among fifty species with more or less conspicuous flowers, twenty-five white, twelve yellow, eight purple or blue, three lilac, and two red or pink; showing a very similar proportion of white and yellow flowers to what obtains further south.

According to the "recent writer" quoted in the first paragraph, what are the two factors that affect light intensity?

Adapted from Common Diseases of Farm Animals by R. A. Craig (1916, 2nd ed.)

The common bot-fly of the horse (G. equi) has a heavy, hairy body. Its color is brown, with dark and yellowish spots. The female fly can be seen during the warm weather, hovering around the horse, and darting toward the animal for the purpose of depositing the egg. The color of the egg is yellow, and it adheres firmly to the hair. It hatches in from two to four weeks, and the larva reaches the mouth through the animal licking the part. From the mouth, it passes to the stomach, where it attaches itself to the gastric mucous membrane. Here it remains until fully developed, when it becomes detached and is passed out with the feces. The third stage is passed in the ground. This takes place in the spring and early summer and lasts for several weeks, when it finally emerges a mature fly.

The bot-fly of the ox (H. lineata) is dark in color and about the size of a honey-bee. On warm days, the female may be seen depositing eggs on the body of the animal, especially in the region of the heels. This seems to greatly annoy the animal, and it is not uncommon for cattle to become stampeded. The egg reaches the mouth through the animal licking the part. The saliva dissolves the shell of the egg and the larva is freed. It then migrates from the gullet, wanders about in the tissue until finally it may reach a point beneath the skin of the back. Here the larva matures and forms the well-known swelling or warble. In the spring of the year it works out through the skin. The next stage is spent in the ground. The pupa state lasts several weeks, when the mature fly issues forth.

The bot-fly of sheep (O. ovis) resembles an overgrown house-fly. Its general color is brown, and it is apparently lazy, flying about very little. This bot-fly makes its appearance when the warm weather begins, and deposits live larvae in the nostrils of sheep. This act is greatly feared by the animals, as shown by their crowding together and holding the head down. The larva works up the nasal cavities and reaches the sinuses of the head, where it becomes attached to the lining mucous membrane. In the spring, when fully developed, it passes out through the nasal cavities and nostrils, drops to the ground, buries itself, and in from four to six weeks develops into the mature fly.

SYMPTOMS OF BOT-FLY DISEASES.—The larvae of the bot-fly of the horse do not cause characteristic symptoms of disease. Work horses that are groomed daily are not hosts for a large number of "bots," but young and old horses that are kept in a pasture or lot and seldom groomed may become unthrifty and "pot bellied," or show symptoms of indigestion.

Cattle suffer much pain from the development of the larva of the H. lineata. During the spring of the year, the pain resulting from the presence of the larvae beneath the skin and the penetration of the skin is manifested by excitement and running about. Besides the loss in milk and beef production, there is a heavy yearly loss from the damage to hides.

The life of the bot-fly of sheep results in a severe catarrhal inflammation of the mucous membrane lining the sinuses of the head, and a discharge of a heavy, pus-like material from the nostrils. The irritation produced by the larvae may be so serious at times as to result in nervous symptoms and death.

Which of the following statements about the bot-fly of the ox is supported by the passage?

"The Cell Cycle" by Joseph Ritchie (2014)

The process by which cells divide and multiply is known as the cell cycle. This cycle consists of two main phases: interphase and mitosis. Each phase consists of a series of clearly defined and observable steps. At the conclusion of the cycle, each parent cell produces two genetically identical daughter cells that may also replicate by proceeding through the cell cycle.

Roughly ninety percent of the cell cycle is spent in interphase. Interphase is comprised of three main steps: the first gap phase, the synthesis phase (also called "S phase"), and the second gap phase. The initial gap phase is a period of cellular preparation in which the cell increases in size and readies itself for DNA synthesis. In the synthesis phase, or S phase, DNA replication occurs, so that when the cell divides, each daughter cell will have the DNA necessary to function properly. In the second gap phase, the cell grows in size and prepares for cellular division in the mitotic phase. At the end of each gap phase, the cell has to pass a regulatory checkpoint to ensure that nothing is going wrong. If anything has gone wrong, the checkpoints stop the cell from proceeding through the cell cycle any further.

The next part of the cell cycle is mitosis. Mitosis is a form of cell division and is broken down into five distinct phases. During prophase, the genetic material contained in the cell’s chromatin condenses into distinct chromosomes. Prometaphase is marked by the breakdown of the cell’s nuclear envelope and the formation of centrosomes at the poles of the cell. During metaphase, the cell’s chromosomes are moved to the center of the cell. A checkpoint ensures that the chromosomes are properly aligned on the center and halts the cell cycle if any errors have occurred. In anaphase, chromosomes break apart at their center, or centromere, and sister chromatids move to opposite ends of the cell. Lastly, telophase and cytokinesis occur as nuclear membranes form to physically divide the cell into two new daughter cells. Chromosomes also unwind into loose chromatin during this part of mitosis. Cytokinesis is defined as the division of the each cell’s cytoplasm and organelles. At the conclusion of the cell cycle, two genetically identical daughter cells have formed.

The cell cycle operates by a series of checkpoints and external cues. This system of checks enables the cell to enter a state of dormancy known as the gap zero phase when conditions or other factors inhibit the cell cycle. Conversely, unregulated and uncontrolled cellular division can occur under certain circumstances. A cell in a state of uncontrolled division is known to be cancerous. Lastly, cells have the ability to mediate their own death by way of apoptosis if certain genetic or physical abnormalities exist. The cell cycle is a complex process that enables cells to replicate and proliferate under a stringent set of checks and balances that produce healthy and viable daughter cells that are each able to perform the process in the future.

How many checkpoints are present in the cell cycle?

"Cacti" by Ami Dave (2013)

Cacti are plants suited to the desert, and we must always keep this factor in mind when growing ornamental cacti in our gardens, for it helps us provide cacti with conditions that allow them to survive and thrive. For example, a cactus should never be watered over its body, as it will start to rot. This is because it is covered with a waxy coating which prevents water loss through evaporation. When one waters the cactus over its body, the waxy coating is washed away and the plant begins to rot. The amount of water that one must supply to the cactus is very much dependent upon the season and upon the climate of the place. During the summer season one should water cacti every four days, whereas in the rainy season, once every fifteen days is quite enough.

Cacti need a minimum of two and a half hours of sunlight per day; however, they should not be kept in the sun all day because they may wrinkle when exposed to too much bright sunlight. Unlike other plants, cacti produce carbon dioxide during the day and oxygen during the night, so they are ideal plants to be kept in bedrooms to freshen up the air at night.

If a cactus is to thrive and prosper, the size of the pot in which it is grown needs to be monitored carefully. The pot should always be a little smaller than the plant itself because it is only when the plant has to struggle to survive that it will thrive. If the pot is too spacious and the plant does not need to struggle, chances are that the cactus will die. Similarly, if a cactus shows no signs of growth, stop watering it. Watering should be resumed only when the plant begins to grow again.

The substrata of a cactus pot is ideally composed of pieces of broken bricks at the bottom, followed by a layer of charcoal above the bricks, and then coarse sand and pebbles above the charcoal. Leaf mould is the best manure.

Grafting cacti is very simple. A very small piece of the cactus plant should be stuck with tape to the plant that needs grafting. The smaller the piece, the easier it is to graft. To reproduce cacti, one has to simply cut off a piece of the cactus, allow it to dry for a few days, and then place it over the cacti substrate. It will automatically develop roots.

It is very easy to differentiate between cacti and other plants that look like cacti. All cacti have fine hair at the base of each thorn. The so-called “thorns” are in fact highly modified leaves which prevent loss of water through transpiration. If one ever gets pricked by cacti thorns, one should take tape, place it over the area where the thorns have penetrated the skin, and then peel it off. All of the thorns will get stuck to the tape and will be removed.

The first paragraph provides all of the following information EXCEPT __________.

"Cacti" by Ami Dave (2013)

Cacti are plants suited to the desert, and we must always keep this factor in mind when growing ornamental cacti in our gardens, for it helps us provide cacti with conditions that allow them to survive and thrive. For example, a cactus should never be watered over its body, as it will start to rot. This is because it is covered with a waxy coating which prevents water loss through evaporation. When one waters the cactus over its body, the waxy coating is washed away and the plant begins to rot. The amount of water that one must supply to the cactus is very much dependent upon the season and upon the climate of the place. During the summer season one should water cacti every four days, whereas in the rainy season, once every fifteen days is quite enough.

Cacti need a minimum of two and a half hours of sunlight per day; however, they should not be kept in the sun all day because they may wrinkle when exposed to too much bright sunlight. Unlike other plants, cacti produce carbon dioxide during the day and oxygen during the night, so they are ideal plants to be kept in bedrooms to freshen up the air at night.

If a cactus is to thrive and prosper, the size of the pot in which it is grown needs to be monitored carefully. The pot should always be a little smaller than the plant itself because it is only when the plant has to struggle to survive that it will thrive. If the pot is too spacious and the plant does not need to struggle, chances are that the cactus will die. Similarly, if a cactus shows no signs of growth, stop watering it. Watering should be resumed only when the plant begins to grow again.

The substrata of a cactus pot is ideally composed of pieces of broken bricks at the bottom, followed by a layer of charcoal above the bricks, and then coarse sand and pebbles above the charcoal. Leaf mould is the best manure.

Grafting cacti is very simple. A very small piece of the cactus plant should be stuck with tape to the plant that needs grafting. The smaller the piece, the easier it is to graft. To reproduce cacti, one has to simply cut off a piece of the cactus, allow it to dry for a few days, and then place it over the cacti substrate. It will automatically develop roots.

It is very easy to differentiate between cacti and other plants that look like cacti. All cacti have fine hair at the base of each thorn. The so-called “thorns” are in fact highly modified leaves which prevent loss of water through transpiration. If one ever gets pricked by cacti thorns, one should take tape, place it over the area where the thorns have penetrated the skin, and then peel it off. All of the thorns will get stuck to the tape and will be removed.

The passage addresses all of the following EXCEPT __________.

Adapted from “In Mammoth Cave” by John Burroughs (1894)

Some idea of the impression which Mammoth Cave makes upon the senses, irrespective even of sight, may be had from the fact that blind people go there to see it, and are greatly struck with it. I was assured that this is a fact. The blind seem as much impressed by it as those who have their sight. When the guide pauses at a more interesting point, or lights the scene up with a great torch or with small flares, and points out the more striking features, the blind exclaim, "How wonderful! How beautiful!" They can feel it, if they cannot see it. They get some idea of the spaciousness when words are uttered. The voice goes forth in these colossal chambers like a bird. When no word is spoken, the silence is of a kind never experienced on the surface of the earth, it is so profound and abysmal. This, and the absolute darkness, to a sighted person makes him feel as if he were face to face with the primordial nothingness. The objective universe is gone; only the subjective remains; the sense of hearing is inverted, and reports only the murmurs from within. The blind miss much, but much remains to them. The great cave is not merely a spectacle to the eye; it is a wonder to the ear, a strangeness to the smell and to the touch. The body feels the presence of unusual conditions through every pore.

Which of the following answer choices is supported by the author’s statement that inside Mammoth Cave, “The objective universe is gone”?

Adapted from “Birds in Retreat” in “Animal Defences—Active Defence” in Volume Four of The Natural History of Animals: The Animal Life of the World in Its Various Aspects and Relations by James Richard Ainsworth Davis (1903)

Among the large running birds are forms, like the African ostrich, in which the absence of powers of flight is largely compensated by the specialization of the legs for the purpose of rapid movement on the ground. For straightforward retreat in open country nothing could be more effective; but another kind of adaptation is required in birds like rails, which are deficient in powers of flight, and yet are able to run through thickly-growing vegetation with such rapidity as to commonly elude their enemies. This is rendered possible by the shape of their bodies, which are relatively narrow and flattened from side to side, so as to easily slip between the stems of grasses, rushes, and similar plants. Anyone who has pursued our native land-rail or corn-crake with intent to capture will have noted how extremely difficult it is even to get within sight of a bird of this sort.

Certain birds, unfortunately for themselves, have lost the power of flight without correspondingly increased powers of running, and have paid the penalty of extinction. Such an arrangement, as might be anticipated, was the result of evolution in islands devoid of any predatory ground-animals, and a classic example of it is afforded by the dodo and its allies, birds related to the pigeons. The dodo itself was a large and clumsy-looking species that at one time abounded in the island of Mauritius, which, like oceanic islands generally, possessed no native mammals, while its indigenous reptiles were only represented by lizards. The ubiquitous sailor, however, and the animals (especially swine) which he introduced, brought about the extinction of this helpless bird in less than a century after its first discovery in 1598. Its memory is now only kept green by a few contemporary drawings and descriptions, certain museum remains, and the proverb "as extinct as a dodo.” A similar fate must overtake any organism suddenly exposed to new and unfavorable conditions, if devoid of sufficient plasticity to rapidly accommodate itself to the altered environment.

What kinds of reptiles are indigenous to Mauritius?

Adapted from “Birds in Retreat” in “Animal Defences—Active Defence” in Volume Four of The Natural History of Animals: The Animal Life of the World in Its Various Aspects and Relations by James Richard Ainsworth Davis (1903)

Among the large running birds are forms, like the African ostrich, in which the absence of powers of flight is largely compensated by the specialization of the legs for the purpose of rapid movement on the ground. For straightforward retreat in open country nothing could be more effective; but another kind of adaptation is required in birds like rails, which are deficient in powers of flight, and yet are able to run through thickly-growing vegetation with such rapidity as to commonly elude their enemies. This is rendered possible by the shape of their bodies, which are relatively narrow and flattened from side to side, so as to easily slip between the stems of grasses, rushes, and similar plants. Anyone who has pursued our native land-rail or corn-crake with intent to capture will have noted how extremely difficult it is even to get within sight of a bird of this sort.

Certain birds, unfortunately for themselves, have lost the power of flight without correspondingly increased powers of running, and have paid the penalty of extinction. Such an arrangement, as might be anticipated, was the result of evolution in islands devoid of any predatory ground-animals, and a classic example of it is afforded by the dodo and its allies, birds related to the pigeons. The dodo itself was a large and clumsy-looking species that at one time abounded in the island of Mauritius, which, like oceanic islands generally, possessed no native mammals, while its indigenous reptiles were only represented by lizards. The ubiquitous sailor, however, and the animals (especially swine) which he introduced, brought about the extinction of this helpless bird in less than a century after its first discovery in 1598. Its memory is now only kept green by a few contemporary drawings and descriptions, certain museum remains, and the proverb "as extinct as a dodo.” A similar fate must overtake any organism suddenly exposed to new and unfavorable conditions, if devoid of sufficient plasticity to rapidly accommodate itself to the altered environment.

According to the passage, which of the following dates could have been the year in which the dodo went extinct?

Adapted from "Recent Views as to Direct Action of Light on the Colors of Flowers and Fruits" in Tropical Nature, and Other Essays by Alfred Russel Wallace (1878)

The theory that the brilliant colors of flowers and fruits is due to the direct action of light has been supported by a recent writer by examples taken from the arctic instead of from the tropical flora. In the arctic regions, vegetation is excessively rapid during the short summer, and this is held to be due to the continuous action of light throughout the long summer days. "The further we advance towards the north, the more the leaves of plants increase in size as if to absorb a greater proportion of the solar rays. M. Grisebach says that during a journey in Norway he observed that the majority of deciduous trees had already, at the 60th degree of latitude, larger leaves than in Germany, while M. Ch. Martins has made a similar observation as regards the leguminous plants cultivated in Lapland.” The same writer goes on to say that all the seeds of cultivated plants acquire a deeper color the further north they are grown, white haricots becoming brown or black, and white wheat becoming brown, while the green color of all vegetation becomes more intense. The flowers also are similarly changed: those which are white or yellow in central Europe becoming red or orange in Norway. This is what occurs in the Alpine flora, and the cause is said to be the same in both—the greater intensity of the sunlight. In the one the light is more persistent, in the other more intense because it traverses a less thickness of atmosphere.

Admitting the facts as above stated to be in themselves correct, they do not by any means establish the theory founded on them; and it is curious that Grisebach, who has been quoted by this writer for the fact of the increased size of the foliage, gives a totally different explanation of the more vivid colors of Arctic flowers. He says, “We see flowers become larger and more richly colored in proportion as, by the increasing length of winter, insects become rarer, and their cooperation in the act of fecundation is exposed to more uncertain chances.” (Vegetation du Globe, col. i. p. 61—French translation.) This is the theory here adopted to explain the colors of Alpine plants, and we believe there are many facts that will show it to be the preferable one. The statement that the white and yellow flowers of temperate Europe become red or golden in the Arctic regions must we think be incorrect. By roughly tabulating the colors of the plants given by Sir Joseph Hooker as permanently Arctic, we find among fifty species with more or less conspicuous flowers, twenty-five white, twelve yellow, eight purple or blue, three lilac, and two red or pink; showing a very similar proportion of white and yellow flowers to what obtains further south.

The “recent writer” quoted in the first paragraph believes that __________.

"Darwin and His Influence" by Joseph Ritchie (2014)

In this passage, “selection” refers to traits that are selected for and passed on to later generations, and “species” refers to organisms that share a common ancestor and can produce viable offspring with one another.

Early in the nineteenth century, scientists sought to understand the differences in the earth’s flora and fauna from their archeological ancestors. The prevailing view at the time was that the differences between current and previous species were unremarkable deviations from their Platonic ideal forms. This theory hinged upon the ideals of the religious-based “created kinds” theory, which state that individuals of today are products of the organisms that were present at the earth’s creation, the result of an intelligent designer. Furthermore, these individuals believed that the differences between organisms could be explained by unseen geological and astrological forces acting on organisms slowly, throughout time. Other scientists also believed that individuals had the ability to change within their lifetimes and pass on traits to their offspring efficiently and quickly through a single generation.

Charles Darwin and other biologists, such as Alfred Wallace, were not greatly influenced by these views and hypotheses. Their propositions stated that species evolve over many generations, due to the selective pressures of their given environments. This evolution could result in the generation of divergent traits, as well as speciation and separation from the original ancestral species. The concept that organisms were not finite or present since creation was very controversial to the scientists of the period. Some saw such an idea as unsupportable, while others perceived it as heretical and fanatical.

Darwin set out to find support for his theory through his work, On the Origins of Species by Means of Natural Selection. He was influenced by archeological discoveries of species, which appeared to have vastly different physiological appearances from present-day organisms. Darwin decided to sail around the world on a Royal Navy ship named the H.M.S. Beagle. During his travels, he was taken to the Pacific islands of the Galapagos archipelago. The volcanic islands followed a patterned distribution on either side of the Equator. The landscapes of each island varied, with different observable flora and fauna. Through scientific observations, Darwin noticed subtle variations of finches on different islands. Some finches had large hard beaks, while others had slender beaks. Beaks were differentiated from island to island. After careful study, Darwin noticed that the beaks seemed to match the food source on each island. The large beaks were specialized for breaking open hard-shelled nuts, while the slender beaks were specialized for eating certain fruits that were abundant. Darwin hypothesized that an ancestral species of finch landed on the islands, and that over generations they became adapted to the locally abundant food sources.

Darwin compiled multiple instances of natural selection and incorporated discoveries made by archeologists and physiologists. He surmised that species evolve over time due to the selective pressures of their respective habitats. These events occur slowly over many generations. Each species selects for advantageous traits among its members. Over time, traits selected as advantageous by environmental pressures and stressors become commonplace in the species. This niche-forming process specializes species by rewarding those with traits most suitable for reproductive success. These traits may progress into speciation of the original species, which results in the eventual development of an entirely new species. Darwin’s theory was met with opposition at the time of its publication, and the theory of evolution remains a controversial topic in several arenas of debate.

According to the passage natural selection allows organisms to perform which of the following statements?

Natural Science: Darwin and his influence by Joseph Ritchie

In this passage, “selection” refers to traits that are selected for and passed on to later generations, and “species” refers to organisms that share a common ancestor and can produce viable offspring with one another.

Early in the nineteenth century, scientists sought to understand the differences in the earth’s flora and fauna from their archeological ancestors. The prevailing view at the time was that the differences between current and previous species were unremarkable deviations from their Platonic ideal forms. This theory hinged upon the ideals of the religious-based “created kinds” theory, which state that individuals of today are products of the organisms that were present at the earth’s creation, the result of an intelligent designer. Furthermore, these individuals believed that the differences between organisms could be explained by unseen geological and astrological forces acting on organisms slowly, throughout time. Other scientists also believed that individuals had the ability to change within their lifetimes and pass on traits to their offspring efficiently and quickly through a single generation.

Charles Darwin and other biologists, such as Alfred Wallace, were not greatly influenced by these views and hypotheses. Their propositions stated that species evolve over many generations, due to the selective pressures of their given environments. This evolution could result in the generation of divergent traits, as well as speciation and separation from the original ancestral species. The concept that organisms were not finite or present since creation was very controversial to the scientists of the period. Some saw such an idea as unsupportable, while others perceived it as heretical and fanatical.

Darwin set out to find support for his theory through his work, On the Origins of Species by Means of Natural Selection. He was influenced by archeological discoveries of species, which appeared to have vastly different physiological appearances from present-day organisms. Darwin decided to sail around the world on a Royal Navy ship named the HMS Beagle. During his travels, he was taken to the Pacific islands of the Galapagos archipelago. The volcanic islands followed a patterned distribution on either side of the Equator. The landscapes of each island varied, with different observable flora and fauna. Through scientific observations, Darwin noticed subtle variations of finches on different islands. Some finches had large hard beaks, while others had slender beaks. Beaks were differentiated from island to island. After careful study, Darwin noticed that the beaks seemed to match the food source on each island. The large beaks were specialized for breaking open hard-shelled nuts, while the slender beaks were specialized for eating certain fruits that were abundant. Darwin hypothesized that an ancestral species of finch landed on the islands, and that over generations they became adapted to the locally abundant food sources.

Darwin compiled multiple instances of natural selection and incorporated discoveries made by archeologists and physiologists. He surmised that species evolve over time due to the selective pressures of their respective habitats. These events occur slowly over many generations. Each species selects for advantageous traits among its members. Over time, traits selected as advantageous by environmental pressures and stressors become commonplace in the species. This niche-forming process specializes species by rewarding those with traits most suitable for reproductive success. These traits may progress into speciation of the original species, which results in the eventual development of an entirely new species. Darwin’s theory was met with opposition at the time of its publication, and the theory of evolution remains a controversial topic in several arenas of debate.

Which of the following statements about the Galapagos is false?

Natural Science: Darwin and his influence by Joseph Ritchie

In this passage, “selection” refers to traits that are selected for and passed on to later generations, and “species” refers to organisms that share a common ancestor and can produce viable offspring with one another.

Early in the nineteenth century, scientists sought to understand the differences in the earth’s flora and fauna from their archeological ancestors. The prevailing view at the time was that the differences between current and previous species were unremarkable deviations from their Platonic ideal forms. This theory hinged upon the ideals of the religious-based “created kinds” theory, which state that individuals of today are products of the organisms that were present at the earth’s creation, the result of an intelligent designer. Furthermore, these individuals believed that the differences between organisms could be explained by unseen geological and astrological forces acting on organisms slowly, throughout time. Other scientists also believed that individuals had the ability to change within their lifetimes and pass on traits to their offspring efficiently and quickly through a single generation.

Charles Darwin and other biologists, such as Alfred Wallace, were not greatly influenced by these views and hypotheses. Their propositions stated that species evolve over many generations, due to the selective pressures of their given environments. This evolution could result in the generation of divergent traits, as well as speciation and separation from the original ancestral species. The concept that organisms were not finite or present since creation was very controversial to the scientists of the period. Some saw such an idea as unsupportable, while others perceived it as heretical and fanatical.

Darwin set out to find support for his theory through his work, On the Origins of Species by Means of Natural Selection. He was influenced by archeological discoveries of species, which appeared to have vastly different physiological appearances from present-day organisms. Darwin decided to sail around the world on a Royal Navy ship named the HMS Beagle. During his travels, he was taken to the Pacific islands of the Galapagos archipelago. The volcanic islands followed a patterned distribution on either side of the Equator. The landscapes of each island varied, with different observable flora and fauna. Through scientific observations, Darwin noticed subtle variations of finches on different islands. Some finches had large hard beaks, while others had slender beaks. Beaks were differentiated from island to island. After careful study, Darwin noticed that the beaks seemed to match the food source on each island. The large beaks were specialized for breaking open hard-shelled nuts, while the slender beaks were specialized for eating certain fruits that were abundant. Darwin hypothesized that an ancestral species of finch landed on the islands, and that over generations they became adapted to the locally abundant food sources.

Darwin compiled multiple instances of natural selection and incorporated discoveries made by archeologists and physiologists. He surmised that species evolve over time due to the selective pressures of their respective habitats. These events occur slowly over many generations. Each species selects for advantageous traits among its members. Over time, traits selected as advantageous by environmental pressures and stressors become commonplace in the species. This niche-forming process specializes species by rewarding those with traits most suitable for reproductive success. These traits may progress into speciation of the original species, which results in the eventual development of an entirely new species. Darwin’s theory was met with opposition at the time of its publication, and the theory of evolution remains a controversial topic in several arenas of debate.

The last paragraph suggests which of the following statements?

"Darwin and His Influence" by Joseph Ritchie (2014)

In this passage, “selection” refers to traits that are selected for and passed on to later generations, and “species” refers to organisms that share a common ancestor and can produce viable offspring with one another.

Early in the nineteenth century, scientists sought to understand the differences in the earth’s flora and fauna from their archeological ancestors. The prevailing view at the time was that the differences between current and previous species were unremarkable deviations from their Platonic ideal forms. This theory hinged upon the ideals of the religious-based “created kinds” theory, which state that individuals of today are products of the organisms that were present at the earth’s creation, the result of an intelligent designer. Furthermore, these individuals believed that the differences between organisms could be explained by unseen geological and astrological forces acting on organisms slowly, throughout time. Other scientists also believed that individuals had the ability to change within their lifetimes and pass on traits to their offspring efficiently and quickly through a single generation.

Charles Darwin and other biologists, such as Alfred Wallace, were not greatly influenced by these views and hypotheses. Their propositions stated that species evolve over many generations, due to the selective pressures of their given environments. This evolution could result in the generation of divergent traits, as well as speciation and separation from the original ancestral species. The concept that organisms were not finite or present since creation was very controversial to the scientists of the period. Some saw such an idea as unsupportable, while others perceived it as heretical and fanatical.

Darwin set out to find support for his theory through his work, On the Origins of Species by Means of Natural Selection. He was influenced by archeological discoveries of species, which appeared to have vastly different physiological appearances from present-day organisms. Darwin decided to sail around the world on a Royal Navy ship named the H.M.S. Beagle. During his travels, he was taken to the Pacific islands of the Galapagos archipelago. The volcanic islands followed a patterned distribution on either side of the Equator. The landscapes of each island varied, with different observable flora and fauna. Through scientific observations, Darwin noticed subtle variations of finches on different islands. Some finches had large hard beaks, while others had slender beaks. Beaks were differentiated from island to island. After careful study, Darwin noticed that the beaks seemed to match the food source on each island. The large beaks were specialized for breaking open hard-shelled nuts, while the slender beaks were specialized for eating certain fruits that were abundant. Darwin hypothesized that an ancestral species of finch landed on the islands, and that over generations they became adapted to the locally abundant food sources.

Darwin compiled multiple instances of natural selection and incorporated discoveries made by archeologists and physiologists. He surmised that species evolve over time due to the selective pressures of their respective habitats. These events occur slowly over many generations. Each species selects for advantageous traits among its members. Over time, traits selected as advantageous by environmental pressures and stressors become commonplace in the species. This niche-forming process specializes species by rewarding those with traits most suitable for reproductive success. These traits may progress into speciation of the original species, which results in the eventual development of an entirely new species. Darwin’s theory was met with opposition at the time of its publication, and the theory of evolution remains a controversial topic in several arenas of debate.

According to evidence in the third paragraph, finches that consume nuts were more likely to possess what kind of beak?

"Darwin and His Influence" by Joseph Ritchie (2014)

In this passage, “selection” refers to traits that are selected for and passed on to later generations, and “species” refers to organisms that share a common ancestor and can produce viable offspring with one another.

Early in the nineteenth century, scientists sought to understand the differences in the earth’s flora and fauna from their archeological ancestors. The prevailing view at the time was that the differences between current and previous species were unremarkable deviations from their Platonic ideal forms. This theory hinged upon the ideals of the religious-based “created kinds” theory, which state that individuals of today are products of the organisms that were present at the earth’s creation, the result of an intelligent designer. Furthermore, these individuals believed that the differences between organisms could be explained by unseen geological and astrological forces acting on organisms slowly, throughout time. Other scientists also believed that individuals had the ability to change within their lifetimes and pass on traits to their offspring efficiently and quickly through a single generation.

Charles Darwin and other biologists, such as Alfred Wallace, were not greatly influenced by these views and hypotheses. Their propositions stated that species evolve over many generations, due to the selective pressures of their given environments. This evolution could result in the generation of divergent traits, as well as speciation and separation from the original ancestral species. The concept that organisms were not finite or present since creation was very controversial to the scientists of the period. Some saw such an idea as unsupportable, while others perceived it as heretical and fanatical.

Darwin set out to find support for his theory through his work, On the Origins of Species by Means of Natural Selection. He was influenced by archeological discoveries of species, which appeared to have vastly different physiological appearances from present-day organisms. Darwin decided to sail around the world on a Royal Navy ship named the H.M.S. Beagle. During his travels, he was taken to the Pacific islands of the Galapagos archipelago. The volcanic islands followed a patterned distribution on either side of the Equator. The landscapes of each island varied, with different observable flora and fauna. Through scientific observations, Darwin noticed subtle variations of finches on different islands. Some finches had large hard beaks, while others had slender beaks. Beaks were differentiated from island to island. After careful study, Darwin noticed that the beaks seemed to match the food source on each island. The large beaks were specialized for breaking open hard-shelled nuts, while the slender beaks were specialized for eating certain fruits that were abundant. Darwin hypothesized that an ancestral species of finch landed on the islands, and that over generations they became adapted to the locally abundant food sources.

Darwin compiled multiple instances of natural selection and incorporated discoveries made by archeologists and physiologists. He surmised that species evolve over time due to the selective pressures of their respective habitats. These events occur slowly over many generations. Each species selects for advantageous traits among its members. Over time, traits selected as advantageous by environmental pressures and stressors become commonplace in the species. This niche-forming process specializes species by rewarding those with traits most suitable for reproductive success. These traits may progress into speciation of the original species, which results in the eventual development of an entirely new species. Darwin’s theory was met with opposition at the time of its publication, and the theory of evolution remains a controversial topic in several arenas of debate.

According to the passage, why did Darwin seek passage on the H.M.S. Beagle?

Adapted from “Humming-Birds: As Illustrating the Luxuriance of Tropical Nature” in Tropical Nature, and Other Essays by Alfred Russel Wallace (1878)

The food of hummingbirds has been a matter of much controversy. All the early writers down to Buffon believed that they lived solely on the nectar of flowers, but since that time, every close observer of their habits maintains that they feed largely, and in some cases wholly, on insects. Azara observed them on the La Plata in winter taking insects out of the webs of spiders at a time and place where there were no flowers. Bullock, in Mexico, declares that he saw them catch small butterflies, and that he found many kinds of insects in their stomachs. Waterton made a similar statement. Hundreds and perhaps thousands of specimens have since been dissected by collecting naturalists, and in almost every instance their stomachs have been found full of insects, sometimes, but not generally, mixed with a proportion of honey. Many of them in fact may be seen catching gnats and other small insects just like fly-catchers, sitting on a dead twig over water, darting off for a time in the air, and then returning to the twig. Others come out just at dusk, and remain on the wing, now stationary, now darting about with the greatest rapidity, imitating in a limited space the evolutions of the goatsuckers, and evidently for the same end and purpose. Mr. Gosse also remarks, ” All the hummingbirds have more or less the habit, when in flight, of pausing in the air and throwing the body and tail into rapid and odd contortions. This is most observable in the Polytmus, from the effect that such motions have on the long feathers of the tail. That the object of these quick turns is the capture of insects, I am sure, having watched one thus engaged pretty close to me.”

What evidence does Mr. Gosse have to support the claim that hummingbirds eat insects?

Adapted from The Effects of Cross & Self-Fertilisation in the Vegetable Kingdom by Charles Darwin (1876)

As it is impossible to exclude such minute pollen-carrying insects as Thrips, flowers which it was intended to fertilise with their own pollen may sometimes have been afterwards crossed with pollen brought by these insects from another flower on the same plant; but as we shall hereafter see, a cross of this kind does not produce any effect, or at most only a slight one. When two or more plants were placed near one another under the same net, as was often done, there is some real though not great danger of the flowers which were believed to be self-fertilised being afterwards crossed with pollen brought by Thrips from a distinct plant. I have said that the danger is not great because I have often found that plants which are self-sterile, unless aided by insects, remained sterile when several plants of the same species were placed under the same net. If, however, the flowers which had been presumably self-fertilised by me were in any case afterwards crossed by Thrips with pollen brought from a distinct plant, crossed seedlings would have been included amongst the self-fertilised; but it should be especially observed that this occurrence would tend to diminish and not to increase any superiority in average height, fertility, etc., of the crossed over the self-fertilised plants.

As the flowers which were crossed were never castrated, it is probable or even almost certain that I sometimes failed to cross-fertilise them effectually, and that they were afterwards spontaneously self-fertilised. This would have been most likely to occur with dichogamous species, for without much care it is not easy to perceive whether their stigmas are ready to be fertilised when the anthers open. But in all cases, as the flowers were protected from wind, rain, and the access of insects, any pollen placed by me on the stigmatic surface whilst it was immature, would generally have remained there until the stigma was mature; and the flowers would then have been crossed as was intended. Nevertheless, it is highly probable that self-fertilised seedlings have sometimes by this means got included amongst the crossed seedlings. The effect would be, as in the former case, not to exaggerate but to diminish any average superiority of the crossed over the self-fertilised plants.

Errors arising from the two causes just named, and from others,—such as some of the seeds not having been thoroughly ripened, though care was taken to avoid this error—the sickness or unperceived injury of any of the plants,—will have been to a large extent eliminated, in those cases in which many crossed and self-fertilised plants were measured and an average struck. Some of these causes of error will also have been eliminated by the seeds having been allowed to germinate on bare damp sand, and being planted in pairs; for it is not likely that ill-matured and well-matured, or diseased and healthy seeds, would germinate at exactly the same time. The same result will have been gained in the several cases in which only a few of the tallest, finest, and healthiest plants on each side of the pots were measured.

Kolreuter and Gartner have proved that with some plants several, even as many as from fifty to sixty, pollen-grains are necessary for the fertilisation of all the ovules in the ovarium. Naudin also found in the case of Mirabilis that if only one or two of its very large pollen-grains were placed on the stigma, the plants raised from such seeds were dwarfed. I was therefore careful to give an amply sufficient supply of pollen, and generally covered the stigma with it; but I did not take any special pains to place exactly the same amount on the stigmas of the self-fertilised and crossed flowers. After having acted in this manner during two seasons, I remembered that Gartner thought, though without any direct evidence, that an excess of pollen was perhaps injurious. It was therefore necessary to ascertain whether the fertility of the flowers was affected by applying a rather small and an extremely large quantity of pollen to the stigma. Accordingly a very small mass of pollen-grains was placed on one side of the large stigma in sixty-four flowers of Ipomoea purpurea, and a great mass of pollen over the whole surface of the stigma in sixty-four other flowers. In order to vary the experiment, half the flowers of both lots were on plants produced from self-fertilised seeds, and the other half on plants from crossed seeds. The sixty-four flowers with an excess of pollen yielded sixty-one capsules; and excluding four capsules, each of which contained only a single poor seed, the remainder contained on an average 5.07 seeds per capsule. The sixty-four flowers with only a little pollen placed on one side of the stigma yielded sixty-three capsules, and excluding one from the same cause as before, the remainder contained on an average 5.129 seeds. So that the flowers fertilised with little pollen yielded rather more capsules and seeds than did those fertilised with an excess; but the difference is too slight to be of any significance. On the other hand, the seeds produced by the flowers with an excess of pollen were a little heavier of the two; for 170 of them weighed 79.67 grains, whilst 170 seeds from the flowers with very little pollen weighed 79.20 grains. Both lots of seeds having been placed on damp sand presented no difference in their rate of germination. We may therefore conclude that my experiments were not affected by any slight difference in the amount of pollen used; a sufficiency having been employed in all cases.

The passage states that which of the following is true?

Adapted from The Effects of Cross & Self-Fertilisation in the Vegetable Kingdom by Charles Darwin (1876)

As it is impossible to exclude such minute pollen-carrying insects as Thrips, flowers which it was intended to fertilise with their own pollen may sometimes have been afterwards crossed with pollen brought by these insects from another flower on the same plant; but as we shall hereafter see, a cross of this kind does not produce any effect, or at most only a slight one. When two or more plants were placed near one another under the same net, as was often done, there is some real though not great danger of the flowers which were believed to be self-fertilised being afterwards crossed with pollen brought by Thrips from a distinct plant. I have said that the danger is not great because I have often found that plants which are self-sterile, unless aided by insects, remained sterile when several plants of the same species were placed under the same net. If, however, the flowers which had been presumably self-fertilised by me were in any case afterwards crossed by Thrips with pollen brought from a distinct plant, crossed seedlings would have been included amongst the self-fertilised; but it should be especially observed that this occurrence would tend to diminish and not to increase any superiority in average height, fertility, etc., of the crossed over the self-fertilised plants.

As the flowers which were crossed were never castrated, it is probable or even almost certain that I sometimes failed to cross-fertilise them effectually, and that they were afterwards spontaneously self-fertilised. This would have been most likely to occur with dichogamous species, for without much care it is not easy to perceive whether their stigmas are ready to be fertilised when the anthers open. But in all cases, as the flowers were protected from wind, rain, and the access of insects, any pollen placed by me on the stigmatic surface whilst it was immature, would generally have remained there until the stigma was mature; and the flowers would then have been crossed as was intended. Nevertheless, it is highly probable that self-fertilised seedlings have sometimes by this means got included amongst the crossed seedlings. The effect would be, as in the former case, not to exaggerate but to diminish any average superiority of the crossed over the self-fertilised plants.

Errors arising from the two causes just named, and from others,—such as some of the seeds not having been thoroughly ripened, though care was taken to avoid this error—the sickness or unperceived injury of any of the plants,—will have been to a large extent eliminated, in those cases in which many crossed and self-fertilised plants were measured and an average struck. Some of these causes of error will also have been eliminated by the seeds having been allowed to germinate on bare damp sand, and being planted in pairs; for it is not likely that ill-matured and well-matured, or diseased and healthy seeds, would germinate at exactly the same time. The same result will have been gained in the several cases in which only a few of the tallest, finest, and healthiest plants on each side of the pots were measured.

Kolreuter and Gartner have proved that with some plants several, even as many as from fifty to sixty, pollen-grains are necessary for the fertilisation of all the ovules in the ovarium. Naudin also found in the case of Mirabilis that if only one or two of its very large pollen-grains were placed on the stigma, the plants raised from such seeds were dwarfed. I was therefore careful to give an amply sufficient supply of pollen, and generally covered the stigma with it; but I did not take any special pains to place exactly the same amount on the stigmas of the self-fertilised and crossed flowers. After having acted in this manner during two seasons, I remembered that Gartner thought, though without any direct evidence, that an excess of pollen was perhaps injurious. It was therefore necessary to ascertain whether the fertility of the flowers was affected by applying a rather small and an extremely large quantity of pollen to the stigma. Accordingly a very small mass of pollen-grains was placed on one side of the large stigma in sixty-four flowers of Ipomoea purpurea, and a great mass of pollen over the whole surface of the stigma in sixty-four other flowers. In order to vary the experiment, half the flowers of both lots were on plants produced from self-fertilised seeds, and the other half on plants from crossed seeds. The sixty-four flowers with an excess of pollen yielded sixty-one capsules; and excluding four capsules, each of which contained only a single poor seed, the remainder contained on an average 5.07 seeds per capsule. The sixty-four flowers with only a little pollen placed on one side of the stigma yielded sixty-three capsules, and excluding one from the same cause as before, the remainder contained on an average 5.129 seeds. So that the flowers fertilised with little pollen yielded rather more capsules and seeds than did those fertilised with an excess; but the difference is too slight to be of any significance. On the other hand, the seeds produced by the flowers with an excess of pollen were a little heavier of the two; for 170 of them weighed 79.67 grains, whilst 170 seeds from the flowers with very little pollen weighed 79.20 grains. Both lots of seeds having been placed on damp sand presented no difference in their rate of germination. We may therefore conclude that my experiments were not affected by any slight difference in the amount of pollen used; a sufficiency having been employed in all cases.

The last paragraph establishes all of the following EXCEPT __________.

Adapted from The Effects of Cross & Self-Fertilisation in the Vegetable Kingdom by Charles Darwin (1876)

As it is impossible to exclude such minute pollen-carrying insects as Thrips, flowers which it was intended to fertilise with their own pollen may sometimes have been afterwards crossed with pollen brought by these insects from another flower on the same plant; but as we shall hereafter see, a cross of this kind does not produce any effect, or at most only a slight one. When two or more plants were placed near one another under the same net, as was often done, there is some real though not great danger of the flowers which were believed to be self-fertilised being afterwards crossed with pollen brought by Thrips from a distinct plant. I have said that the danger is not great because I have often found that plants which are self-sterile, unless aided by insects, remained sterile when several plants of the same species were placed under the same net. If, however, the flowers which had been presumably self-fertilised by me were in any case afterwards crossed by Thrips with pollen brought from a distinct plant, crossed seedlings would have been included amongst the self-fertilised; but it should be especially observed that this occurrence would tend to diminish and not to increase any superiority in average height, fertility, etc., of the crossed over the self-fertilised plants.

As the flowers which were crossed were never castrated, it is probable or even almost certain that I sometimes failed to cross-fertilise them effectually, and that they were afterwards spontaneously self-fertilised. This would have been most likely to occur with dichogamous species, for without much care it is not easy to perceive whether their stigmas are ready to be fertilised when the anthers open. But in all cases, as the flowers were protected from wind, rain, and the access of insects, any pollen placed by me on the stigmatic surface whilst it was immature, would generally have remained there until the stigma was mature; and the flowers would then have been crossed as was intended. Nevertheless, it is highly probable that self-fertilised seedlings have sometimes by this means got included amongst the crossed seedlings. The effect would be, as in the former case, not to exaggerate but to diminish any average superiority of the crossed over the self-fertilised plants.

Errors arising from the two causes just named, and from others,—such as some of the seeds not having been thoroughly ripened, though care was taken to avoid this error—the sickness or unperceived injury of any of the plants,—will have been to a large extent eliminated, in those cases in which many crossed and self-fertilised plants were measured and an average struck. Some of these causes of error will also have been eliminated by the seeds having been allowed to germinate on bare damp sand, and being planted in pairs; for it is not likely that ill-matured and well-matured, or diseased and healthy seeds, would germinate at exactly the same time. The same result will have been gained in the several cases in which only a few of the tallest, finest, and healthiest plants on each side of the pots were measured.

Kolreuter and Gartner have proved that with some plants several, even as many as from fifty to sixty, pollen-grains are necessary for the fertilisation of all the ovules in the ovarium. Naudin also found in the case of Mirabilis that if only one or two of its very large pollen-grains were placed on the stigma, the plants raised from such seeds were dwarfed. I was therefore careful to give an amply sufficient supply of pollen, and generally covered the stigma with it; but I did not take any special pains to place exactly the same amount on the stigmas of the self-fertilised and crossed flowers. After having acted in this manner during two seasons, I remembered that Gartner thought, though without any direct evidence, that an excess of pollen was perhaps injurious. It was therefore necessary to ascertain whether the fertility of the flowers was affected by applying a rather small and an extremely large quantity of pollen to the stigma. Accordingly a very small mass of pollen-grains was placed on one side of the large stigma in sixty-four flowers of Ipomoea purpurea, and a great mass of pollen over the whole surface of the stigma in sixty-four other flowers. In order to vary the experiment, half the flowers of both lots were on plants produced from self-fertilised seeds, and the other half on plants from crossed seeds. The sixty-four flowers with an excess of pollen yielded sixty-one capsules; and excluding four capsules, each of which contained only a single poor seed, the remainder contained on an average 5.07 seeds per capsule. The sixty-four flowers with only a little pollen placed on one side of the stigma yielded sixty-three capsules, and excluding one from the same cause as before, the remainder contained on an average 5.129 seeds. So that the flowers fertilised with little pollen yielded rather more capsules and seeds than did those fertilised with an excess; but the difference is too slight to be of any significance. On the other hand, the seeds produced by the flowers with an excess of pollen were a little heavier of the two; for 170 of them weighed 79.67 grains, whilst 170 seeds from the flowers with very little pollen weighed 79.20 grains. Both lots of seeds having been placed on damp sand presented no difference in their rate of germination. We may therefore conclude that my experiments were not affected by any slight difference in the amount of pollen used; a sufficiency having been employed in all cases.

Which of the following statements about the plants is supported by the passage?

Adapted from The Effects of Cross & Self-Fertilisation in the Vegetable Kingdom by Charles Darwin (1876)

As it is impossible to exclude such minute pollen-carrying insects as Thrips, flowers which it was intended to fertilise with their own pollen may sometimes have been afterwards crossed with pollen brought by these insects from another flower on the same plant; but as we shall hereafter see, a cross of this kind does not produce any effect, or at most only a slight one. When two or more plants were placed near one another under the same net, as was often done, there is some real though not great danger of the flowers which were believed to be self-fertilised being afterwards crossed with pollen brought by Thrips from a distinct plant. I have said that the danger is not great because I have often found that plants which are self-sterile, unless aided by insects, remained sterile when several plants of the same species were placed under the same net. If, however, the flowers which had been presumably self-fertilised by me were in any case afterwards crossed by Thrips with pollen brought from a distinct plant, crossed seedlings would have been included amongst the self-fertilised; but it should be especially observed that this occurrence would tend to diminish and not to increase any superiority in average height, fertility, etc., of the crossed over the self-fertilised plants.

As the flowers which were crossed were never castrated, it is probable or even almost certain that I sometimes failed to cross-fertilise them effectually, and that they were afterwards spontaneously self-fertilised. This would have been most likely to occur with dichogamous species, for without much care it is not easy to perceive whether their stigmas are ready to be fertilised when the anthers open. But in all cases, as the flowers were protected from wind, rain, and the access of insects, any pollen placed by me on the stigmatic surface whilst it was immature, would generally have remained there until the stigma was mature; and the flowers would then have been crossed as was intended. Nevertheless, it is highly probable that self-fertilised seedlings have sometimes by this means got included amongst the crossed seedlings. The effect would be, as in the former case, not to exaggerate but to diminish any average superiority of the crossed over the self-fertilised plants.

Errors arising from the two causes just named, and from others,—such as some of the seeds not having been thoroughly ripened, though care was taken to avoid this error—the sickness or unperceived injury of any of the plants,—will have been to a large extent eliminated, in those cases in which many crossed and self-fertilised plants were measured and an average struck. Some of these causes of error will also have been eliminated by the seeds having been allowed to germinate on bare damp sand, and being planted in pairs; for it is not likely that ill-matured and well-matured, or diseased and healthy seeds, would germinate at exactly the same time. The same result will have been gained in the several cases in which only a few of the tallest, finest, and healthiest plants on each side of the pots were measured.

Kolreuter and Gartner have proved that with some plants several, even as many as from fifty to sixty, pollen-grains are necessary for the fertilisation of all the ovules in the ovarium. Naudin also found in the case of Mirabilis that if only one or two of its very large pollen-grains were placed on the stigma, the plants raised from such seeds were dwarfed. I was therefore careful to give an amply sufficient supply of pollen, and generally covered the stigma with it; but I did not take any special pains to place exactly the same amount on the stigmas of the self-fertilised and crossed flowers. After having acted in this manner during two seasons, I remembered that Gartner thought, though without any direct evidence, that an excess of pollen was perhaps injurious. It was therefore necessary to ascertain whether the fertility of the flowers was affected by applying a rather small and an extremely large quantity of pollen to the stigma. Accordingly a very small mass of pollen-grains was placed on one side of the large stigma in sixty-four flowers of Ipomoea purpurea, and a great mass of pollen over the whole surface of the stigma in sixty-four other flowers. In order to vary the experiment, half the flowers of both lots were on plants produced from self-fertilised seeds, and the other half on plants from crossed seeds. The sixty-four flowers with an excess of pollen yielded sixty-one capsules; and excluding four capsules, each of which contained only a single poor seed, the remainder contained on an average 5.07 seeds per capsule. The sixty-four flowers with only a little pollen placed on one side of the stigma yielded sixty-three capsules, and excluding one from the same cause as before, the remainder contained on an average 5.129 seeds. So that the flowers fertilised with little pollen yielded rather more capsules and seeds than did those fertilised with an excess; but the difference is too slight to be of any significance. On the other hand, the seeds produced by the flowers with an excess of pollen were a little heavier of the two; for 170 of them weighed 79.67 grains, whilst 170 seeds from the flowers with very little pollen weighed 79.20 grains. Both lots of seeds having been placed on damp sand presented no difference in their rate of germination. We may therefore conclude that my experiments were not affected by any slight difference in the amount of pollen used; a sufficiency having been employed in all cases.

Which of the following most fully lists errors named by the author in the third paragraph?