Bacteria and Types of Prokaryotes

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MCAT Biology › Bacteria and Types of Prokaryotes

Questions 1 - 10
1

The presence of which of the following feature would best indicate a eukaryotic cell?

Organelles

Ribosomes

Plasma membrane

Homeostasis

Cilia

Explanation

Organelles are only found in eukaryotic cells which set them apart from prokaryotic cells.

2

Which of the following is found in bacterial cells, but not in mature red blood cells?

DNA

Cell membrane

Mitochondria

Nucleus

Explanation

Bacterial cells are prokaryotic and lack all membrane-bound organelles. We can thus eliminate mitochondria and nucleus as answers. These structures are not found in bacterial cells or in mature erythrocytes.

All cells, regardless of origin, are surrounded by a cell membrane. This structure is present in both prokaryotes and erythrocytes.

DNA is used to code for the genome of the organism and is found in both prokaryotes and eukaryotes. Bacteria contain a cytsolic genome known as the nucleoid. Red blood cells, however, lack DNA. This is partly due to their lack of a nucleus, and is considered evolutionarily advantageous because no viruses can target red blood cells.

3

Which of the following produces ATP without utilizing oxygen, even when oxygen is available?

Aerotolerant organisms

Facultative anaerobes

Obligate aerobes

Obligate anaerobes

Microaerophiles

Explanation

Anaerobic environments lack oxygen, while aerobic environments contain oxygen. Generally, aerobic respiration is more energetically efficient than anaerobic respiration mechanisms. Aerotolerant organisms, however, will continue to use anaerobic means of energy production, even in the presence of oxygen. These are a special class of organism that can survive in either an aerobic or anaerobic environment.

Obligate anaerobes require an anaerobic environment and find oxygen to be toxic. These organisms will not be able to produce ATP in the presence of oxygen. Obligate aerobes require the presence of oxygen in order to survive and cannot produce ATP without oxygen utilization. Facultative anaerobes can switch between metabolism mechanisms, using cellular respiration in the presence of oxygen and fermentation or anaerobic respiration in its absence. Microaerophiles are aerobic organisms, but require a particularly low level of oxygen in order to survive.

4

Which of the following is not a characteristic of prokaryotes?

Presence of mitochondria

Circular chromosome

Presence of a cell wall

Presence of ribosomes

Explanation

Prokaryotes do not have membrane-bound organelles, such as the nucleus or mitochondria. In fact, endosymbiotic theory suggests that mitochondria evolved from symbiotic prokaryotic relationships. Prokaryotes are often associated with all of the other characteristics listed.

5

Sexually transmitted diseases are a common problem among young people in the United States. One of the more common diseases is caused by the bacterium Neisseria gonorrhoeae, which leads to inflammation and purulent discharge in the male and female reproductive tracts.

The bacterium has a number of systems to evade host defenses. Upon infection, it uses pili to adhere to host epithelium. The bacterium also uses an enzyme, gonococcal sialyltransferase, to transfer a sialyic acid residue to a gonococcal surface lipooligosaccharide (LOS). A depiction of this can be seen in Figure 1. The sialyic acid residue mimics the protective capsule found on other bacterial species.

Once infection is established, Neisseria preferentially infects columnar epithelial cells in the female reproductive tract, and leads to a loss of cilia on these cells. Damage to the reproductive tract can result in pelvic inflammatory disease, which can complicate pregnancies later in the life of the woman.

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A scientist is using a Gram stain to investigate Neisseria. If Neisseria is bright pink upon examination after staining, which of the following is likely true?

Neisseria is Gram negative, and has reduced peptidoglycan on its surface

Neisseria is Gram positive, and has reduced peptidoglycan on its surface

Neisseria is Gram negative, and has abundant peptidoglycan on its surface

Neisseria is Gram positive, and has abundant peptidoglycan on its surface

Neisseria only has a cell wall, and no underlying cell membrane

Explanation

Bright pink cells under Gram stain are characteristic of Gram negative status. This is associated with an outer lipid membrane and reduced peptidoglycan. The reduction in peptidoglycan is what leads to a failure to retain the Gram stain, which would have colored the cells purple.

6

Prokaryotes that can utilize oxygen if available, but do not require it, are called __________.

facultative anaerobes

constitutive anaerobes

obligate anaerobes

obligate aerobes

constitutive aerobes

Explanation

Facultative anaerobes are adaptive prokaryotes that can survive in either aerobic or anaerobic environments. These organisms are capable of both cellular respiration, through use of the electron transport chain along the cell membrane, and fermentation. They will adopt the more efficient process (cellular respiration) when oxygen is present, but can easily switch between one metabolism and the other.

Obligate anaerobes cannot produce energy in the presence of oxygen, and obligate aerobes cannot produce energy in the absence of oxygen.

7

All of the following are true about bacilli bacteria except:

I. All bacilli are rod-shaped

II. All bacilli are gram positive

III. All bacilli are aerobic

III only

II only

I and III

II and III

Explanation

Bacilli is a genus of bacteria that are characterized by their rod-shaped cells. Bacilli can be either gram positive, such as Bacillus anthracis, or gram negative, such as Salmonella enterica. Bacilli bacteria can be either aerobic (require oxygen) or facultative anaerobes (can thrive in both oxygen rich and oxygen poor environment). Bacilli are not exclusively aerobic, nor are they exclusively gram positive.

8

Compared to gram negative bacteria, how many more membrane-bound organelles do gram positive bacteria contain?

Zero

One

Two

Three

Explanation

The main difference between a gram positive and a gram negative bacterial species is the thickness of their cell walls: gram positive bacteria have thicker cell walls than gram negative bacteria. The interior of each bacterium, however, contains the same types of structures. Recall that bacteria, in general, have no membrane-bound organelles; therefore, both gram positive and gram negative bacteria will have zero membrane-bound organelles. The internal structures of each bacterial class are essentially indistinguishable.

9

Which of the following cell types does not have a cell wall?

Trichocyte (forms fur and nails)

Fungal cell

Plant cell

Gram negative bacteria

Gram positive bacteria

Explanation

No animal cells will have cell walls. Trichocytes are specialized to be hard and resilient, but do not have cell walls.

Fungi, plants, and bacteria do use cell walls. The distinction between gram positive and gram negative bacteria refers to the composition of the cell wall only; both positive and negative bacteria have a cell wall present.

10

Eukaryotes can share which of the following structures with prokaryotes?

Cell wall

Mitochondria

Golgi body

Nucleoid

Explanation

The chief difference between prokaryotic and eukaryotic cells is the presence of membrane-bound organelles. Mitochondria and Golgi bodies are examples of such organelles, and are only found in eukaryotes. Nucleoids are only found in prokaryotes, while eukaryotes use membrane-bound nuclei.

Cell walls can be found in either prokaryotes or eukaryotes. Many prokaryotic bacteria use cell walls as protection from the external environment, while eukaryotic plants cells use cell walls to help maintain rigidity and prevent water loss.

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