Understanding Ribosomes

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GRE Subject Test: Biology › Understanding Ribosomes

Questions 1 - 3
1

Ribosomes are the organelle responsible for the translation of mRNAs into proteins. To do this, ribosomes translate the mRNA codons into amino acids which are joined to form a long polypeptide. The ribosome has a catalytic domain that is responsible for the formation of the peptide bonds between these amino acids. What is the name, i.e. the enzymatic classification, of this domain in the ribosome?

Peptidyl transferase

Peptidyl acylase

AminoATPase

Polymerase

Ester transferase

Explanation

Interestingly, ribosomes are one of the only examples of an RNA structure that has enzymatic activity. The primary enzymatic function of the ribosome is one of a peptidyl transferase; that is, the catalysis of peptide bond formation between amino acids as those acids are brought to the nascent strand.

2

Which of the following best describes the ribosome in terms of its structural components?

RNA-protein complex

DNA-protein complex

Multimeric protein complex

RNA monomer

Protein subunit

Explanation

Ribosomes can be classified as ribonucleoproteins because the proteins are associated with ribonucleic acids (RNA). Thus, RNA-protein complex is the best descriptor.

3

Which of the following is true about eukaryote ribosomes?

Eukaryote ribosomes are 80S, with one large 60S subunit and one small 40S subunit

Eukaryote ribosomes are 80S, with one large 50S subunit and one small 30S subunit

Eukaryote ribosomes are 70S, with one large 60S subunit and one small 40S subunit

Eukaryote ribosomes are 80S, with two large 60S subunits and one small 40S subunit

Eukaryote ribosomes are 80S, with one large 50S subunit and one small 40S subunit

Explanation

Eukaryotic ribosomes are 80S with one large 60S subunit and one small 40S subunit. Prokaryotic ribosomes are 70S, with one large 50S subunit and one small 30S subunit. "S" refers to the sedimentation coefficient (Svedberg), which is a particles sedimentation velocity for a given applied acceleration.

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