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Questions 1 - 10
1

What is the likely genetic make-up of a virus which contains a RNA-dependent RNA polymerase?

Minus-strand RNA

Plus-strand RNA

Single-stranded DNA

Double-stranded DNA

Double-stranded RNA

Explanation

We're given the type of enzyme contained within a virus, and we're asked to make a determination of the virus' genetic makeup.

To begin with, we're told that the enzyme is an RNA-dependent RNA polymerase. The name of the enzyme gives us insight into what it does. It requires RNA as a template to produce more RNA.

So if this enzyme can convert RNA into RNA, where does the original RNA come from? The answer is that it must come from the virus. This means that we must be dealing with single-stranded RNA.

Now, the question is to determine the sense of the RNA genome of the virus. That is to say, it can be minus or plus. A minus-sense RNA is one whose complementary sequence can be translated into protein. A plus-sense RNA is one that doesn't need any processing to be translated. Rather, plus-sense RNA can be translated right away. Since we know that the enzyme present is going to produce RNA from RNA, we can then reason that the viral genome is likely minus-sense. When the minus-sense RNA is enacted on by this enzyme, the result is a new strand of RNA that can be translated into protein to serve the needs of the virus.

2

Which enzyme could be considered to violate the "central dogma" of biology?

Reverse transcriptase

Helicase

Catalase

Carboxylase

Ligase

Explanation

The "central dogma" of biology says that information goes from DNA via transcription to RNA via translation to proteins. Reverse transcriptases, however, employed by retroviruses, synthesize DNA from RNA. As for the other enzymes: one function of helicases (among others) is to pull apart double helix strands. Catalase breaks down hydrogen peroxide. Carboxylase adds a carboxyl group to a substrate, and a ligase creates a bond between two molecules, for example, via a phosphodiester bond.

3

Which of the following are true regarding the product of transcription?

I. The product has more uracil than the parent strand

II. The product has both hydrophilic and hydrophobic amino acids

III. The nucleotides on the RNA polymerase binds to complementary nucleotides on the parent strand

I only

II only

I and II

II and III

Explanation

Transcription is the second process involved in the production of proteins from a gene. The three processes are DNA replication, transcription, and translation. DNA replication involves replication of DNA from a parent strand, transcription involves the synthesis of a RNA molecule from a DNA molecule, and translation involves the conversion of the mRNA molecule to a polypeptide.

As mentioned, transcription produces an RNA molecule from a DNA molecule (parent strand). Recall that RNA molecules have uracil, whereas DNA molecules have thymine; therefore, the product will contain more uracil.

Amino acids are found in proteins. Since the products of transcription are nucleic acids (RNA molecules) they won’t contain any amino acids. Recall that a nucleic acid consists of pentose sugar molecules (ribose in RNA and deoxyribose in DNA), nitrogenous bases (adenine, guanine, cytosine, thymine (in DNA), and uracil (in RNA)), and phosphate groups.

RNA polymerase is an important enzyme involved in transcription. Its function is to add nucleotides to the growing mRNA chain. Although it adds complementary nucleotides to the DNA, RNA polymerase itself doesn’t bind to complementary DNA sequences, rather it binds at promoters.

4

A researcher is analyzing an enzyme. Results reveal that the enzyme is found in eukaryotes, and is involved in synthesis of ribosomes. Which of the following could be the identity of the enzyme?

RNA polymerase I

RNA polymerase II

RNA polymerase III

More than one of these could be correct

Explanation

There are three types of RNA molecules. First, mRNA molecules are the main products of transcription that undergo translation to produce most of the proteins found in a cell. Second, tRNA molecules are special RNA molecules that facilitate the addition of amino acids to a growing polypeptide chain during translation. Third, rRNA molecules are components of ribosomes and are synthesized in the nucleolus (location of assembly of ribosomes). The enzyme in this question is involved in the production of rRNA molecules. RNA polymerase I is used in production of rRNA molecules. RNA polymerase II is used for mRNA molecules and RNA polymerase III is used for tRNA molecules.

5

Which of the following matches is correct regarding eukaryotic termination of transcription?

The poly A tail is added to the 3' end of mRNA

The poly A tail is added to the 5' end of mRNA

The mRNA cap is added to the 3' end of mRNA

The mRNA cap and the poly A tail are added to the 5' end of mRNA

The mRNA cap and the Poly A tail are added to the 3' end of mRNA

Explanation

After transcription, mRNA is modified so that it can be preserved for a longer time in the cell. A nucleotide cap structure is attached to the 5' end of the mRNA and a poly A tail is attached to the 3' end of the mRNA in order to accomplish this goal.

6

Within a eukaryotic chromosome, which of the following pairs of processes can occur at the same time?

Transcription and splicing

Replication and transcription

Transcription and translation

Translation and splicing

Replication and splicing

Explanation

In eukaryotes, transcription and splicing could occur simultaneously. Both of these processes take place in the nucleus of the cell, while translation takes place in the cytoplasm. Therefore, translation could not happen at the same time as either transcription or splicing. Replication occurs totally independently from all of the other processes listed.

7

What is the relationship of the RNA transcript to the DNA template strand?

I. The RNA transcript is antiparallel to the DNA template strand.

II. The RNA transcript is complementary to the DNA template strand.

III. The RNA transcript is identical to the DNA template strand.

IV. In the RNA transcript, thymine is replaced with uracil.

I, II, and IV

I and IV

I, II, and III

I, III, and IV

I and II

Explanation

The RNA transcript contains nucleotide bases at each position, which are complementary to the DNA. RNA is synthesized in the 5' to 3' direction from a DNA template strand with antiparallel direction (3' to 5').The coding DNA strand is identical to the RNA transcript with the exception that thymine is replaced with uracil in RNA.

8

Which of the following is a ribozyme?

RNase P

RNA polymerase II

Guanyltransferase

RNase H

All of these are ribozymes

Explanation

RNase H is involved in reverse transcriptase, but it is not a ribozyme. RNase P is a ribonuclease that cleaves/processes rRNA and generates 5' ends. The RNA component of RNase P is its catalytic subunit.

9

Reverse transcriptase is an enzyme that produces DNA molecules from RNA. Which of the following is correct?

All of these

Retroviruses have this enzyme and use it to replicate their RNA genome

Telomerase (the enzyme that maintains telomere length in eukaryotes) has reverse transcriptase activity

In eukaryotes reverse transcriptase is encoded by retrotransposons

Reverse transcriptase activity is present in DNA viruses

Explanation

The reverse transcriptase activity of retroviruses like HIV is used to replicate their RNA genome in the host cell. This activity is not needed or present in DNA viruses which can use the host's enzymes to replicate. The reverse transcriptase activity of telomerases is used to prevent telomere ends shortening after multiple replications in somatic cells. Telomeres are short sequences at the end of chromosomes that prevent deterioration of the chromosomes. Retrotransposones are elements that amplify repetitive sequences in the DNA of eukaryotes.

10

Oftentimes, biochemists say that the genetic code is degenerate. What are they referring to when they say this?

Some amino acids can be coded for by more than just one codon

A change in the reading frame alters expression of all subsequent codons

Some codons are able to code for more than just one amino acid

The sequence of one type of molecule is able to code for the sequence of another type of molecule

The same genetic code is not shared by all species

Explanation

While some of these answer choices are true, others are false. But even for the choices that are true, only one of them directly answers the question. Let's go ahead and look at each choice.

  • Some amino acids can be coded for by more than just one codon

This is a true statement, and is also the correct answer. The degeneracy of the code is due to the fact that, for some amino acids, a number of different codons can result in the same amino acid. For example, the amino acid tyrosine can be coded for by either UAU or by UAC.

  • A change in the reading frame alters expression of all subsequent codons

While this is a true statement, it does not answer the question. Nonetheless, it's important to know that mutations which either insert or delete a nucleotide will change the entire rest of the reading frame. Consequently, there are likely to be many errors and the resultant polypeptide will likely not be functional.

  • Some codons are able to code for more than just one amino acid

This is a false statement. But be careful. This answer choice looks a lot like the correct one. The difference is that, for this choice, we're talking about a single codon being able to code for more than one amino acid. This is not the case.

  • The sequence of one type of molecule is able to code for the sequence of another type of molecule

This is a true statement with regards to gene expression. For transcription, the DNA (sequence of deoxyribonucleotides) serves as a template for the formation of mRNA (sequence of ribonucleotides). And for translation, that same mRNA serves as a precursor for the formation of a polypeptide (sequence of amino acids).

  • The same genetic code is not shared by all species

From what we know, this is a false statement. The genetic code is universal, meaning that all living things have been found to have the same codon-amino acid relationship.

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