Biochemistry › pH Regulation
What is the concentration in an
solution with a pH of
?
Here is the equation needed to find the correct answer to this question.
Is water an acid or a base?
Both
Acid
Base
It is impossible to predict.
Water, , is an amphoteric substance--it can act as either an acid or a base. In certain circumstances, water can act as a Bronsted-Lowry acid by donating a proton.
As seen above, donated one of its hydrogen atoms, becoming
.
In other cases, water can act as a Bronsted-Lowry base by accepting a proton.
As seen above, accepted a hydrogen atom to become
.
Therefore, water can act as either an acid or a base depending on the situation. There are other amphoteric substances, but water is definitely the most common.
Calculate the pH of an ammonia buffer when the molar ratio of is
. The pKa to be used is 9.75.
This question requires you to use the Henderson-Hasselbach equation, one of the most important equations in biochemistry. The equation is:
where is the concentration of the conjugate base, and
is the concentration of the acid. In this scenario,
is the conjugate base, while
is the acid. With the numbers given in this question, the equation should look like this:
What is the pOH of a solution of
?
Here are the equations we need to use to find the pOH of our solution of sulfuric acid:
If a patient's lab values from the doctor's office show a blood plasma pH of 7.1, which of the following could be the correct diagnosis?
Respiratory acidosis
Respiratory alkalosis
The results show normal blood plasma pH.
Metabolic alkalosis
Normal blood pH is 7.4. A decrease in pH could indicate acidosis, which is associated with too much in the blood. Respiratory acidosis occurs as the result of the lungs failing to eliminate enough
.
Listed below are the pKa values of five common biochemical buffers.
Formic acid: 3.75
Acetic acid: 4.76
2-(N-Morpholino)ethanesulfonic acid (MES): 6.09
Tris(hydroxymethyl)aminomethane (Tris): 8.08
Glycine: 9.78
Which of the following would have the best buffering capacity in a solution with pH 4.0?
Formic acid
Acetic acid
MES
Tris
Glycine
The best buffering capacity occurs when pH = pKa. When this is true, the ratio of ionized to unionized form of the buffer is 1:1. Thus the solution can best resist changes in pH, as hydrogen ions can be quenched or donated to solution to resist change. Acetic acid would have almost the same buffering capacity since its pKa is almost as close to 4.0 as that of formic acid.
What is buffering capacity?
The extent to which a buffer can counteract the effect of added acid or base
The molecular weight of the substance used as a buffer
The pH of a buffer solution
The effectiveness of commercial antacids
The amount of buffer present in a solution
Buffering capacity refers to how well a buffer works. A buffer is a substance that maintains a specific pH regardless of added acid or base. Thus, buffering capacity refers to how well a buffer maintains the pH of a solution despite the the effects of added acid or base. The other choices do not apply to this definition.
If the pH of blood is considered to be 7.4 and the pKa of a compound in the blood is 6.4, what is the ratio of the acid form of the compound to the base form of the compound?
Using the Henderson Hasselbach equation:
Thus, the ratio of acid to base =
What is the pOH of a 0.5 M solution?
Recall the equation for pH. Here is the calculation that should be performed:
Upon running lab tests, you determine that a patient has very low blood pH. Which of the following could have caused this low pH?
Hypoventilation
Hyperventilation
Increased red blood cells
Increased iron in blood
Low blood pH suggests that the patient has high concentration of hydrogen ions. To solve this question, we need to look at the following reaction, which represents the major blood buffer system:
One way the body controls the amount of hydrogen ions in the blood is by altering the amount of carbon dioxide. Recall that, according to Le Chatelier’s principle, increasing carbon dioxide will push the reaction the right and increase hydrogen ion concentration whereas decreasing carbon dioxide will decrease hydrogen ion concentration.
Body controls carbon dioxide levels via breathing. Hyperventilation refers to increased breathing whereas hypoventilation refers to decreased breathing. During hyperventilation the person breathes out excess carbon dioxide (decreasing the hydrogen ion concentration). During hypoventilation, on the other hand, a person breathes slowly and retains carbon dioxide (increasing the hydrogen ion concentration). The patient in this question has low blood pH (high hydrogen ion concentration); therefore, of the options, the patient must be hypoventilating.