Identifying Specific Nucleic Acid Structures

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Biochemistry › Identifying Specific Nucleic Acid Structures

Questions 1 - 10
1

Cytosine

What is the name of the molecule shown?

Cytosine

Cytidine

Uracil

Thymine

Pyrimidine

Explanation

A nucleoside is the nitrogenous base bound to a sugar via glycosidic bond. A nucleotide is a nitrogenous base bonded to a sugar bonded to the phosphate. This nitrogenous base is cytosine. If it were bound to ribose, it would be called cytidine. Although cytosine is a pyrimidine like uracil and thymine, pyrimidine is a class of ring structure, not the specific name of a base.

2

Which of the following are pyrimidines?

Cytosine (C), uracil (U), and thymine (T)

Adenine (A) and guanine (G)

Cytosine (C) and uracil (U)

Adenine (A), guanine (G), and uracil (U)

Explanation

Think of it as this. CUT the PY (pie) PYrimidines. PURe As Gold. PURines. A will pair with T (and U in RNA). G will pair with C. The deamination of cytosine makes uracil, which is in RNA.

3

In the eukaryotic ribosome, the sedimentation coefficient for the small subunit is __________ and large subunit is __________.

40S . . . 60S

20S . . . 60S

60S . . . 80S

35S . . . 70S

40S . . . 70S

Explanation

The 80S eukaryotic ribosome is made up of 40S and 60S subunits.

4

Adenine

Identify the given nucleotide, nucleoside or nitrogenous base.

Adenine

Guanine

Adenosine

Guanosine

Purine

Explanation

This nitrogenous base is adenine. Although it has a purine ring system, purine is not its specific name. If the adenine were bound to a sugar, it would be called adenosine.

5

Pyrimidine and purine

Name the given ring systems and determine whether they are soluble or insoluble.

Pyrimidine ring; purine ring; insoluble

Pyrimidine ring; purine ring; soluble

Purine ring; pyrimidine ring; insoluble

Cytosine; adenine,; soluble

Amine ring; insoluble

Explanation

The six-membered ring is a pyrimidine. The purine ring system has two rings: a six-membered and a five-membered ring. These aromatic rings are hydrophobic, making them relatively insoluble. The pyrimidine bases in nucleotides are cytosine, uracil, and thymine; the purine bases in nucleotides are adenine and guanine.

6

Which of the following are considered pyrimidines?

I: adenine

II: thymine

III: cytosine

IV: guanine

II and III

I and II

III and IV

I and IV

I and III

Explanation

Pyrimidines are nitrogenous bases with 1 ring structure, whereas purines are nitrogenous bases with 2 ring structures. Cytosine and thymine are pyrimidines since they both have one ring structure, whereas adenine and guanine are purines with two connected ring structures. (Note: in RNA, uracil would be considered a pyrimidine in the place of thymine.) One way to help you remember which bases belong to which chemical groups, remember that pyrimidines, like pyramids are sharp, and sharp things CUT - Cytosine, Uracil, and Thymine.

7

Identify the nucleobase.

Adenine

Adenine

Guanine

Cytosine

Thymine

Uracil

Explanation

The structure is adenine because of the characteristic amine group on carbon 6 and lack of any other substituents.

8

Compared to an RNA molecule, a DNA molecule has __________ thymine molecules and __________ deoxygenated sugars.

more . . . more

fewer . . . more

more . . . fewer

fewer . . . fewer

Explanation

DNA and RNA molecules contain four types of nitrogenous bases. Guanine, cytosine and adenine are found in both whereas thymine is only found in DNA and uracil is only found in RNA. This means that DNA molecules will have more Thymine bases than RNA molecules. Another difference between DNA and RNA molecule is the type of pentose sugar. RNA contains ribose sugar whereas DNA contains deoxyribose. Deoxyribose contains one less hydroxyl group than ribose; therefore, DNA molecules have more deoxygenated sugars than RNA.

9

Which of the following nitrogenous bases is found exclusively in DNA?

Uracil

Uracil

Cytosine

Adenine

Adenine

Explanation

Thymine is the nitrogenous base that is only found in DNA. All of the other bases can be found in both DNA and RNA.

10

Adenosine

Identify the given nucleotide, nucleoside or nitrogenous base.

Adenosine

Adenine

Purine

Guanine

Guanosine

Explanation

While adenine is the name of this nitrogenous base of the purine family, when bonded to ribose, a nucleoside is formed. This nucleoside is called adenosine. A nucleoside consists of a nitrogenous base and a pentose sugar (either ribose or deoxyribose). A nucleotide consists of a nitrogenous base, a pentose sugar, and at least one phosphate group.

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