Biochemistry
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Amino terminal - Ala - Lys - Glu - Phe - Phe - Ala - Leu - carboxyl terminal.
If the above primary sequence is cleaved by trypsin, on which amino acid will the new amino terminal be?
Glu
Lys
Phe
Ala
Leu
Explanation
Trypsin will cleave the primary sequence after the lysine residue (on its carboxyl side). Thus, Lys will be the new carboxyl terminal and Glu will be the new amino terminal. Remember that a protein's primary sequence is written from N to C.
What is the role of ubiquinone in the electron transport chain?
Ubiquinone carries electrons from the first enzyme complex to the second enzyme complex
Ubiquinone carries electrons from the third enzyme complex to the fourth enzyme complex
Ubiquinone accepts electrons directly from
Ubiquinone accepts electrons directly from
Ubiquinone is the final step in which oxygen is reduced to water
Explanation
Ubiquinone functions to carry electrons in oxidative phosphorylation from the first enzyme complex to the second enzyme complex. It does not receive electrons from nor
directly.
If an uncoupler allows for excess buildup of protons inside of the mitochondrial matrix, which of the following processes will be inhibited?
None of these will be inhibited
Pyruvate dehydrogenase complex
Electron transport chain
Glycolysis
Krebs cycle
Explanation
With the excess buildup of protons in the matrix, the only thing that will be inhibited is the generation of ATP by ATP synthase. The other processes in cellular respiration focus more on creation of high energy electron carriers, and therefore will continue as normal.
Which of the following lists these molecules in order of increasing permeability to a lipid bilayer?
Sodium ion, glucose, water, carbon dioxide
Carbon dioxide, water, glucose, sodium ion
Glucose, carbon dioxide, sodium ion, water
Glucose, carbon dioxide, water, sodium ion
Sodium ion, glucose, carbon dioxide, water
Explanation
Charged molecules do not permeate the lipid bilayer easily at all. So despite its small size, among our choices, a sodium ion passes least easily through. Polar molecules also have a hard (but less difficult) time passing through, and the larger the molecule, the harder that becomes, so after the sodium ion comes glucose, followed by water, which is polar but much smaller. Small, hydrophobic molecules -- such as carbon dioxide -- diffuse through most easily, because they can pass through the longest (hydrophobic) part of the membrane.
Cholesterol has a structure most similar to which of these?
Steroid
Phospholipid
Glycolipid
Carbohydrate
Free fatty acid
Explanation
Although it is one of the three main types of membrane lipids (along with phospholipids and glycolipids) cholesterol's structure is quite different. It is a steroid, and so it is made from four linked hydrocarbon rings, including three six-membered rings and one five-membered ring.
Which of the following metabolic processes directly requires oxygen?
Electron transport system
Glycolysis
Citric acid cycle
Tricarboxylic acid cycle
Pyruvate dehydrogenase complex
Explanation
The electron transport system is the only metabolic process listed that directly requires molecular oxygen. Oxygen is the final electron acceptor (it is one of the most electronegative atoms in our bodies) in the electron transport chain. This is the same as saying that oxygen has the highest reduction potential, and is capable of receiving electons. If oxygen is not present to accept the electron from the final enzyme complex in the inner mitochondrial membrane, then electron transport will be inhibited and thus no ATP will be produced via chemiosmosis.
Note that the Krebs cycle, citric acid cycle, and tricarboxylic acid cycle (TCA cycle) all refer to the same process, and do not directly require oxygen (oxygen is neither a reactant nor a product in any of the steps). However, oxygen is indirectly required, as there is no point to this cycle without subsequent oxidative phosphorylation. Thus in the absence of oxygen, of the choices shown, only glycolysis will proceed uninhibited.
If an uncoupler allows for excess buildup of protons inside of the mitochondrial matrix, which of the following processes will be inhibited?
None of these will be inhibited
Pyruvate dehydrogenase complex
Electron transport chain
Glycolysis
Krebs cycle
Explanation
With the excess buildup of protons in the matrix, the only thing that will be inhibited is the generation of ATP by ATP synthase. The other processes in cellular respiration focus more on creation of high energy electron carriers, and therefore will continue as normal.
Which of the following best explains a quaternary structure of a protein?
Hydrogen and disulfide bonds, two or more polypeptide chains
Hydrogen bonds, alpha-helices and beta-pleated sheets
Linear sequence of amino acids
Disulfide bonds, single polypeptide chain
Collagen and myoglobin
Explanation
Primary structure: linear sequence of amino acids
Secondary structure: hydrogen bonds, alpha-helices and beta-pleated sheets
Tertiary structure: disulfide bonds, single polypeptide chain
Myoglobin is a monomer, and is made of a single polypeptide chain. Thus, its highest level of protein structure is tertiary. While collagen does contain different polypeptide chains, it is an example of a protein with quaternary structure, not an explanation of what this means.
Which of these lipid molecules has a different fundamental structure than the others?
Cholesterol
Fatty acids
Glycolipids
Sphingolipids
Triglyceride
Explanation
Cholesterol is made up of multiple rings, including three six-carbon rings and one five-carbon ring. This characteristic structure is also seen in steroid hormones and metabolites, as many biologically relevant molecules are derived from cholesterol. Fatty acids are long hydrocarbons (typically between ten and thirty carbons long) with carboxylic acid functional groups on one end. Glycolipids are lipids that have carbohydrate moieties attached, which play a role in cellular and molecular communication. Sphingolipids are a class of lipids that contain a sphingoid base backbone and include sphingosine, sphingomyelin, ceramides, gangliosides and others.
Select the most accurate description of alpha-linolenic acid.
Is a precursor for eicosapentaenoic acid (EPA)
Can be synthesized de novo in the body
Is a twenty-carbon chain omega-3 fatty acid
Is a precursor for arachidonic acid (AA)
Is an eighteen-carbon chain omega-6 fatty acid
Explanation
Alpha-linolenic acid is an essential fatty acid that must be consumed in the diet (cannot be synthesized by the body). It is an eighteen-carbon omega-3 fatty acid that is used to synthesize eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), two important long chain polyunsaturated fatty acids. Alpha-linoleic acid is the precursor to arachodonic acid (AA).