Understanding Mitochondria and Chloroplasts

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Biology › Understanding Mitochondria and Chloroplasts

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1

Which of the following organelles is commonly referred to as the powerhouse of the cell?

Mitochondria

Golgi apparatus

Rough endoplasmic reticulum

Lysosome

Nucleus

Explanation

The mitochondria are responsible for synthesis of the majority of the cell's ATP. This is why mitochondria are commonly referred to as the powerhouse of the cell. The rough endoplasmic reticulum and Golgi apparatus are involved in protein synthesis and delivery. The nucleus houses the DNA and is the site of transcription and ribosome assembly (nucleolus). The lysosome contains hydrolytic enzymes that are used for degradation of certain materials.

2

Which of the following organelles contain their “own” DNA?

All of these

Nuclei

Chloroplasts

Mitochondria

Explanation

All of the listed organelles contain DNA. Scientists have identified nuclear DNA, mitochondrial DNA and chloroplast DNA that are exclusive to these cellular structures.

3

Where does the Krebs cycle take place in the mitochondria?

Mitochondrial matrix

Outer mitochondrial membrane

Inner mitochondrial membrane

Intermembrane space

Explanation

The mitochondria are the site for aerobic respiration in the cell. Both the Krebs cycle and electron transport chain are found in the mitochondria, while glycolysis (anaerobic metabolism) takes place in the cytoplasm. The Krebs cycle takes place in the mitochondrial matrix, where pyruvate is used to generate NADH and FADH2. These molecules are then taken to the inner mitochondrial membrane, where the electron transport chain is located. The electron transport chain pushes protons into the intermembrane space, creating the proton gradient that fuels ATP synthesis.

4

How is mitochondrial DNA (mDNA) different from nuclear DNA?

All of these answers

mDNA is circular rather than linear

mDNA is inherited solely from the mother

mDNA does not have introns

mDNA does not have histones

Explanation

Mitochondrial DNA is much smaller and simpler than nuclear DNA, meaning it does not need histones or introns. Mitochondrial DNA is also circular, like bacterial DNA, rather than linear like nuclear DNA. As the ovum is the only contributor of mitochondria to an offspring, all mDNA must therefore be inherited from the mother.

5

All of the following are true of chloroplasts except __________.

they are found in animal cells

they are found in plant cells

They contain tiny pigments called chlorophylls

The chemical energy that they produce is used to make sugars

Explanation

Chloroplasts are the organelles found in plant cells that contain chlorophyll pigments which conduct photosynthesis in plants , some bacteria, and some protists. They form glucose molecules, which can be connected to form polysaccharides of starch. Animal cells do not have chloroplasts. Rather, animals need to eat their food, they cannot produce their own food from the sun.

6

The mitochondria are commonly considered the powerhouses of the cell. The energy (ATP) necessary for cell function is created within the mitochondria via the process of oxidative phosphorylation (the electron transport chain). In order for oxidative phosphorylation to be effective, certain proteins must maintain a proton gradient between the two membranes that surround the mitochondria.

Studying the structure of the mitochondria, you will notice that the organelle has two membranes. The outer membrane is smooth, while the inner membrane has numerous inversions and folds. These folds are called cristae. What is the purpose of the cristae?

The cristae provide greater surface area for the inner mitochondrial membrane, allowing more electron transport proteins in the organelle and more energy to be produced

No known function exists for the cristae.

The cristae serve to funnel protons deeper into the organelle, aiding in oxidative phosphorylation

The folds are a byproduct of the highly acidic environment caused by the proton gradient. They exist to prevent the proton gradient from damaging the outer mitochondrial membrane.

They serve as the location where proteins involved in glycolysis reside

Explanation

The cristae increase the surface area available within the cell. In order for oxidative phosphorylation of occur, certain proteins must be embedded in the inner mitochondrial membrane. The fold in the membrane allow for a larger surface area, which in turn allows more of these proteins to be embedded. More proteins to create energy means more energy (ATP) for the cell.

7

Which of the following cellular organelles is responsible for making a majority of the cell's energy?

Mitochondria

Rough endoplasmic reticulum

Lysosomes

Golgi apparatus

Nucleus

Explanation

Mitochondria produces most of the energy of the cell. The nucleus stores genetic information and is the site of transcription. The rough endoplasmic reticulum is the site of translation of proteins that have destinations other than in the cytoplasm. These proteins made in the rough endoplasmic reticulum are subsequently transported to the Golgi apparatus, where they are modified, packaged, and sent to their final destinations. Lysosomes have an acidic environment in which digestive enzymes break down polymers that will eventually be recycled.

8

Which of the following is not true regarding mitochondria and chloroplasts?

Only mitochondria are thought to have arisen via endosymbiosis

Chloroplasts are only found in photosynthetic cells, whereas mitochondria are found in animal and plant cells

Chloroplasts contain many pigment molecules that absorb sunlight, while mitochondria do not absorb sunlight

The inner membrane of mitochondria forms cristae and the inner membrane of chloroplasts form thylakoids

Explanation

Mitochondria and chloroplasts are plastids, and are thought to have arisen in eukaryotic cells via endosymbiosis. All the other options are true of mitochondria and chloroplasts.

9

What is the "powerhouse" of the cell?

Mitochondria

Nucleus

Ribosome

Cell membrane

Cell wall

Explanation

The mitochondria is responsible for making the energy for the cell by cellular respiration. It does this by taking the major breakdown products of glucose (pyruvate and NADH) and converting them into ATP by the citric acid cycle and electron transport chain. This is an oxygen-dependent process.

10

What organelle is associated with photosynthesis in algae?

Chloroplasts

Mitochondria

Plasmids

Guard cells

Explanation

Chloroplasts are an organelle that harbors large amounts of the green pigment chlorophyll. The chloroplast converts the energy of sunlight into chemical energy through a process called photosynthesis. This takes place inside a system of membranous sacs called thylakoids within the chloroplast.

Plants, algae, and cyanobacteria all produce energy via photosynthesis. Plants and algae use chloroplasts, but cyanobacteria are prokaryotes and do not contain membrane-bound organelles. Cyanobacteria use internal thylakoid structures to perform photosynthesis.

Plasmids are circular pieces of DNA found in prokaryotes, and are not linked to photosynthesis. Guard cells are responsible for opening and closing stomata on the surface of plant leaves, and are also not involved in photosynthesis.

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