Bacteria

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NCLEX-PN › Bacteria

Questions 1 - 10
1

Which agar would be the best choice option to identify_Escherichia coli_?

EMB (eosin methylene blue), because it is selective and differential

Nutrient agar

Selenium enriched media

Phenethyl alcohol

Explanation

The question requires the knowledge that Escherichia coli is a facultative, Gram negative, lactose fermenting bacteria. EMB (eosin methylene blue), or Mac (MacConkey) media is superior for isolating Escherichia coli because it is both selective and differential. Nutrient agar is neither selective nor differential. Selenium enriched media would not help identify this bacteria because Escherichia coli does not require this supplemented media to grow. Phenethyl alcohol can be used to select for anaerobic bacteria, which is of no utility in this situation.

2

Which agar would be the best choice option to identify_Escherichia coli_?

EMB (eosin methylene blue), because it is selective and differential

Nutrient agar

Selenium enriched media

Phenethyl alcohol

Explanation

The question requires the knowledge that Escherichia coli is a facultative, Gram negative, lactose fermenting bacteria. EMB (eosin methylene blue), or Mac (MacConkey) media is superior for isolating Escherichia coli because it is both selective and differential. Nutrient agar is neither selective nor differential. Selenium enriched media would not help identify this bacteria because Escherichia coli does not require this supplemented media to grow. Phenethyl alcohol can be used to select for anaerobic bacteria, which is of no utility in this situation.

3

What is the most common infection preceding the development of Guillain-Barré syndrome?

Campylobacter jejuni

Salmonella

E. coli

S_taphylococcus aureus_

Explanation

30% of cases of Guillain-Barré are preceded by infection by C_ampylobacter jejuni._ There is no known association between S_taphylococcus aureus, Salmonella,_ or E.coli and Guillain-Barré syndrome.

4

What is the most common infection preceding the development of Guillain-Barré syndrome?

Campylobacter jejuni

Salmonella

E. coli

S_taphylococcus aureus_

Explanation

30% of cases of Guillain-Barré are preceded by infection by C_ampylobacter jejuni._ There is no known association between S_taphylococcus aureus, Salmonella,_ or E.coli and Guillain-Barré syndrome.

5

All of the following are frequently implicated in epiglottitis except __________.

Campylobacter jejuni

H. influenzae type B

Staphylococcus aureus

Streptococcus spp

Explanation

The most commonly implicated bacteria in epiglottiits are H. influenzae type B, Streptococcus spp, and Staphylococcus aureus. _Campylobacter jejuni is a common cause of food poisoning.

6

All of the following are frequently implicated in epiglottitis except __________.

Campylobacter jejuni

H. influenzae type B

Staphylococcus aureus

Streptococcus spp

Explanation

The most commonly implicated bacteria in epiglottiits are H. influenzae type B, Streptococcus spp, and Staphylococcus aureus. _Campylobacter jejuni is a common cause of food poisoning.

7

Conjugation between bacteria is carried out via what structure?

Pili

Flagella

Cilia

Capsule

Explanation

Pili are short, hairlike structures on the surface of some bacteria. Conjugative pili can form a sort of tube between bacterial cells, allowing for the transfer of DNA from one bacteria to another.

Flagella are whip-like structures that allows bacteria to move within a fluid medium. Cilia are hair-like structures found primarily on the surface of mammalian cells, but are also found on bacterial cells, where they aid in attachment. A bacterial capsule is a polysaccharide layer outside the cell envelope that does not take part in bacterial conjugation, and may secrete "slime" which protects the bacteria from the surrounding environment.

8

Conjugation between bacteria is carried out via what structure?

Pili

Flagella

Cilia

Capsule

Explanation

Pili are short, hairlike structures on the surface of some bacteria. Conjugative pili can form a sort of tube between bacterial cells, allowing for the transfer of DNA from one bacteria to another.

Flagella are whip-like structures that allows bacteria to move within a fluid medium. Cilia are hair-like structures found primarily on the surface of mammalian cells, but are also found on bacterial cells, where they aid in attachment. A bacterial capsule is a polysaccharide layer outside the cell envelope that does not take part in bacterial conjugation, and may secrete "slime" which protects the bacteria from the surrounding environment.

9

A synthetic protein is created inside a bacterium from an introduced RNA strand. Which method does the bacterium employ to create the desired protein?

Translation

Replication

Transcription

Transduction

Explanation

This question requires that the student recognizes that the process illustrated is recombinant DNA (deoxyribonucleic acid) technique. It requires the understanding that the process of producing protein from RNA (ribonucleic acid) is termed translation. Replication is the process by which DNA is synthesized from DNA. Transcription proceeds translation and involves synthesizing RNA transcripts of specific sections of DNA. Transduction is one way bacteria exchange genetic information. Specifically, transduction involves the transfer of bacterial DNA via bacteriophage. Note that this is one way that a group of bacteria can become resistant to antibiotics without ever having been exposed to the antibiotic to which they are resistant.

10

A synthetic protein is created inside a bacterium from an introduced RNA strand. Which method does the bacterium employ to create the desired protein?

Translation

Replication

Transcription

Transduction

Explanation

This question requires that the student recognizes that the process illustrated is recombinant DNA (deoxyribonucleic acid) technique. It requires the understanding that the process of producing protein from RNA (ribonucleic acid) is termed translation. Replication is the process by which DNA is synthesized from DNA. Transcription proceeds translation and involves synthesizing RNA transcripts of specific sections of DNA. Transduction is one way bacteria exchange genetic information. Specifically, transduction involves the transfer of bacterial DNA via bacteriophage. Note that this is one way that a group of bacteria can become resistant to antibiotics without ever having been exposed to the antibiotic to which they are resistant.

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