The correct answer is polymerase cycling assembly. In this reaction, multiple template sequences are included into a reaction with primers to the 5' most and 3' most sequence of the desired final product. The template sequences must also have regions of homology overlap with each other such that upon denaturing and annealing they hybridize to form a larger fragment. Upon hybridization, the primers will promote polymerase amplification of one large product.

The correct answer is polymerase cycling assembly. In this reaction, multiple template sequences are included into a reaction with primers to the 5' most and 3' most sequence of the desired final product. The template sequences must also have regions of homology overlap with each other such that upon denaturing and annealing they hybridize to form a larger fragment. Upon hybridization, the primers will promote polymerase amplification of one large product.

Running allozyme gels was one of the first methods used in molecular studies of these enzymes that have slightly different functions due to having slightly different genetic codes (alleles). This term refers specifically to enzymatic variation created by genetic variation/alleles.

Running allozyme gels was one of the first methods used in molecular studies of these enzymes that have slightly different functions due to having slightly different genetic codes (alleles). This term refers specifically to enzymatic variation created by genetic variation/alleles.

For this problem we need to determine the equation of our standard curve. This can be done by creating a graph from the data points and determining the slope.

Assuming that a sample of zero concentration will also have zero absorbance, we can find the equation for the line generated by finding the slope.

Pick two points on the line to find the slope. We will use (0,0) and (40,0.405).

Use this equation and the absorbance given in the question to find the concentration of the unknown sample.

For this problem we need to determine the equation of our standard curve. This can be done by creating a graph from the data points and determining the slope.

Assuming that a sample of zero concentration will also have zero absorbance, we can find the equation for the line generated by finding the slope.

Pick two points on the line to find the slope. We will use (0,0) and (40,0.405).

Use this equation and the absorbance given in the question to find the concentration of the unknown sample.

If he cannot generate a mutant, it is most likely because the mutant phenotype is lethal. In theory, this researcher was successful in mutating the target gene, however the result of the mutation was a failure during development. This would prevent any potential offspring with the mutation from developing, resulting in no viable specimens for the study.

Many proteins are conserved throughout evolution, but can still be easily mutated. For example, the gene for the protein hemoglobin is well-preserved between animal species, but can still be manipulated to generate animal models for various disorders. The distinction between heterochromatin and euchromatin doesn't really explain why the researcher would be unable to generate a mutant.

If he cannot generate a mutant, it is most likely because the mutant phenotype is lethal. In theory, this researcher was successful in mutating the target gene, however the result of the mutation was a failure during development. This would prevent any potential offspring with the mutation from developing, resulting in no viable specimens for the study.

Many proteins are conserved throughout evolution, but can still be easily mutated. For example, the gene for the protein hemoglobin is well-preserved between animal species, but can still be manipulated to generate animal models for various disorders. The distinction between heterochromatin and euchromatin doesn't really explain why the researcher would be unable to generate a mutant.

When tagging the N-terminus of a protein, the main purpose is to detect this protein by a widely commercial antibody to the tag. Many proteins that researchers intend to study do not have a specific antibody, and therefore, the utilization of a HA or FLAG tag allows easy detection. The tag is not intended to alter the native protein's function nor change its expression level in cells.

When tagging the N-terminus of a protein, the main purpose is to detect this protein by a widely commercial antibody to the tag. Many proteins that researchers intend to study do not have a specific antibody, and therefore, the utilization of a HA or FLAG tag allows easy detection. The tag is not intended to alter the native protein's function nor change its expression level in cells.