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Lipids and Biological Membranes (5D) Practice Test
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Q1
Investigators reconstituted a planar phospholipid bilayer from purified lipids and monitored lateral diffusion of a fluorescent lipid analog (a proxy for membrane fluidity) in a chamber containing 150 mM NaCl. When temperature was increased from 20°C to 37°C, diffusion increased markedly. The group then repeated the experiment with 30 mol% cholesterol incorporated into the bilayer. Which change is most consistent with cholesterol’s role in membrane structure across this temperature shift?
Investigators reconstituted a planar phospholipid bilayer from purified lipids and monitored lateral diffusion of a fluorescent lipid analog (a proxy for membrane fluidity) in a chamber containing 150 mM NaCl. When temperature was increased from 20°C to 37°C, diffusion increased markedly. The group then repeated the experiment with 30 mol% cholesterol incorporated into the bilayer. Which change is most consistent with cholesterol’s role in membrane structure across this temperature shift?