Chiral Separation and Enantiomer Resolution (5C) Practice Test

A racemic chiral alcohol is analyzed by gas chromatography (GC) using an achiral capillary column at 1 atm with a temperature program that fully elutes the compound. A single GC peak is observed. The same sample analyzed by chiral HPLC shows two peaks. Based on the technique used, which conclusion is most consistent with the results?

A chiral drug is administered as a racemate, but only the (S)-enantiomer binds a target receptor in vivo. A manufacturing team uses chiral HPLC to isolate (S) with 99% enantiomeric excess (ee) prior to formulation. If the final dose contains 100 mg total drug substance, what is most consistent with the meaning of 99% ee for this sample?