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Ions in Solutions (5A) Practice Test
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Q1
A protein purification step uses a cation-exchange resin bearing fixed negative sulfonate groups (Resine). A sample in low-salt buffer contains Naba, Cababa, and a positively charged peptide Pba. The resin is initially in the Naba form. Ion exchange can be represented as: Resineb7Naba + Pba cc Resineb7Pba + Naba. Which outcome would be expected when the NaCl concentration in the buffer is increased substantially during elution?
A protein purification step uses a cation-exchange resin bearing fixed negative sulfonate groups (Resine). A sample in low-salt buffer contains Naba, Cababa, and a positively charged peptide Pba. The resin is initially in the Naba form. Ion exchange can be represented as: Resineb7Naba + Pba cc Resineb7Pba + Naba. Which outcome would be expected when the NaCl concentration in the buffer is increased substantially during elution?