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Translation and Post-Translational Modification (1B) Practice Test

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Q1

A research group expressed a receptor-associated adaptor protein (Adap1) in mammalian cells. After stimulation with a growth factor, Adap1 shifted to a slower-migrating band on SDS-PAGE, and the shift was eliminated by phosphatase treatment. A mutant Adap1 in which a single serine in a consensus kinase motif was replaced with alanine failed to show the shift and showed prolonged receptor signaling. Based on the findings, what effect would the modification have on Adap1 function?

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