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Nucleic Acid Structure and Base Pairing (1B) Practice Test
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Q1
A 16-bp DNA hairpin is engineered with a 6-bp stem and a 4-nt loop. In one variant, two A–T base pairs in the stem are replaced with two G–C base pairs while keeping the stem length constant and maintaining perfect complementarity. Under identical buffer conditions, what effect would this mutation most likely have on the hairpin’s stem stability?
A 16-bp DNA hairpin is engineered with a 6-bp stem and a 4-nt loop. In one variant, two A–T base pairs in the stem are replaced with two G–C base pairs while keeping the stem length constant and maintaining perfect complementarity. Under identical buffer conditions, what effect would this mutation most likely have on the hairpin’s stem stability?