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Regulation of Gene Expression in Eukaryotes (1B) Practice Test
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Q1
A lab tests whether a putative silencer element (S) represses transcription of the eukaryotic gene GATA4. A luciferase reporter is driven by the GATA4 minimal promoter (P) alone or by P plus S cloned upstream. Cells are transfected with either an empty vector or a plasmid expressing the transcription factor REST. Results after 24 hours are shown:
Condition 1: P only + empty vector → 100% luciferase
Condition 2: P+S + empty vector → 95% luciferase
Condition 3: P only + REST → 90% luciferase
Condition 4: P+S + REST → 25% luciferase
Based on the setup, which outcome is most likely if S is mutated so that REST can no longer bind, while all other sequences remain unchanged?
A lab tests whether a putative silencer element (S) represses transcription of the eukaryotic gene GATA4. A luciferase reporter is driven by the GATA4 minimal promoter (P) alone or by P plus S cloned upstream. Cells are transfected with either an empty vector or a plasmid expressing the transcription factor REST. Results after 24 hours are shown:
Condition 1: P only + empty vector → 100% luciferase
Condition 2: P+S + empty vector → 95% luciferase
Condition 3: P only + REST → 90% luciferase
Condition 4: P+S + REST → 25% luciferase
Based on the setup, which outcome is most likely if S is mutated so that REST can no longer bind, while all other sequences remain unchanged?