Microbiology Quiz
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CLINICAL AND DIAGNOSTIC MICROBIOLOGY
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PASSAGE
A cerebrospinal fluid (CSF) sample is tested for JC virus DNA. The initial conventional PCR is negative. The laboratory then performs a second PCR using the product of the first reaction as a template and a new set of primers that target a region within the first amplicon. This second PCR is positive.
What is the primary diagnostic advantage of performing this nested PCR procedure described in the passage?
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