Electrophoresis and Protein Separation (5C)
MCAT · Flashcards · Application
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QUESTION
In IEF, if a protein is at a region where
pH
>
p
I
\text{pH} > pI
pH
>
p
I
, which direction will it migrate?
QUESTION
In IEF, if a protein is at a region where
pH
<
p
I
\text{pH} < pI
pH
<
p
I
, which direction will it migrate?
QUESTION
If
E
E
E
doubles while gel and sample are unchanged, what happens to velocity
v
v
v
if
μ
\mu
μ
is constant?
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ANSWER
v
v
v
doubles because
v
=
μ
E
v = \mu E
v
=
μ
E
. Velocity is directly proportional to electric field at constant mobility.
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