Chromatography Techniques (5C)
MCAT · Flashcards · Application
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QUESTION
Identify which has larger
k
′
k'
k
′
: analyte with
t
R
=
6
min
t_R = 6\,\text{min}
t
R
=
6
min
or
t
R
=
3
min
t_R = 3\,\text{min}
t
R
=
3
min
if
t
M
=
1
min
t_M = 1\,\text{min}
t
M
=
1
min
.
QUESTION
Calculate
R
f
R_f
R
f
if a TLC spot travels
3.0
cm
3.0\,\text{cm}
3.0
cm
and the solvent front travels
6.0
cm
6.0\,\text{cm}
6.0
cm
.
QUESTION
Identify which has larger
k
′
k'
k
′
: analyte with
t
R
=
6
min
t_R = 6\,\text{min}
t
R
=
6
min
or
t
R
=
3
min
t_R = 3\,\text{min}
t
R
=
3
min
if
t
M
=
1
min
t_M = 1\,\text{min}
t
M
=
1
min
.
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ANSWER
Analyte with
t
R
=
6
min
t_R = 6\,\text{min}
t
R
=
6
min
has larger
k
′
k'
k
′
. Longer retention time yields a higher capacity factor, as
k
′
k'
k
′
increases with greater
(
t
R
−
t
M
)
(t_R - t_M)
(
t
R
−
t
M
)
.
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