In normal-phase TLC on silica, which compound travels farther: less polar or more polar?
QUESTION
What is the key separation principle of affinity chromatography?
QUESTION
What is reversed-phase chromatography defined by regarding stationary and mobile phase polarity?
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ANSWER
Nonpolar stationary phase with relatively polar mobile phase. This setup reverses the polarity gradient, enabling separation based on hydrophobicity rather than polarity.