Protein Folding, Stability, and Denaturation (1A)
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QUESTION
Which bond type is directly broken by proteolysis but not by typical denaturation: peptide or hydrogen bond?
QUESTION
Which noncovalent interaction primarily stabilizes an
α
\alpha
α
-helix and a
β
\beta
β
-sheet backbone?
QUESTION
Identify the term for the set of all conformations a protein can sample during folding.
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ANSWER
The conformational ensemble. Proteins fluctuate among various states, with the native form being the most populated under equilibrium conditions.
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