Enzyme Structure and Catalytic Mechanisms (1A)
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QUESTION
What is the transition state in an enzyme-catalyzed reaction?
QUESTION
What is a cofactor in enzyme function?
QUESTION
What does a low
K
m
K_m
K
m
indicate about an enzyme for its substrate?
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ANSWER
High apparent affinity (less substrate needed to reach
V
m
a
x
2
\frac{V_{max}}{2}
2
V
ma
x
). Lower
K
m
K_m
K
m
means stronger binding, requiring less substrate for significant activity.
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