Chem & Physics Quiz
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A sequencing facility reports that a particular 12-bp DNA duplex has an unusually high melting temperature compared with another 12-bp duplex of similar length and salt conditions. Duplex X contains 9 G·C pairs and 3 A·T pairs; Duplex Y contains 3 G·C pairs and 9 A·T pairs. Which statement is most consistent with the nucleotide structure described and best explains why Duplex X has higher Tm?
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