Chem & Physics Quiz
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A lab separated two proteins (P and Q) using native agarose gel electrophoresis at constant voltage (120 V) with the same gel concentration. The buffer pH was varied.
Observed migration toward the anode (+) from the cathode (−):
- At pH 5.5: P migrated 0.4 cm; Q migrated 3.6 cm
- At pH 8.5: P migrated 4.2 cm; Q migrated 0.6 cm
Which conclusion about their isoelectric points (pI) is most consistent with the data?
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