Bio & Biochem Quiz
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FOUNDATIONAL CONCEPT 3: ORGAN SYSTEMS AND HOMEOSTASIS
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In a study of hepatocytes, investigators perfused cells with a peptide hormone (H) that does not cross the plasma membrane. Within 60 seconds, intracellular cAMP increased ~6-fold and phosphorylation of a cytosolic enzyme increased. When cells were pretreated with a competitive antagonist to the H receptor, both the cAMP rise and enzyme phosphorylation were abolished. The investigators note that H circulates largely unbound in plasma and has a short half-life.
Which outcome would be expected if the receptor is blocked, as in the antagonist condition?
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