Bio & Biochem Quiz
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FOUNDATIONAL CONCEPT 2: CELLS AND CELLULAR ORGANIZATION
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A hospital lab tracks ciprofloxacin resistance in a clonal population of Gram-negative bacteria. After 24 hours of growth in sublethal ciprofloxacin, survivors show a 50-fold increase in MIC. Sequencing reveals a single recurrent point mutation in the gene encoding DNA gyrase; no new plasmids are detected and conjugation is prevented. When the mutant gyrase is purified, ciprofloxacin binding is markedly reduced compared with wild-type. Which genetic change is most consistent with the resistance observed?
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