Bio & Biochem Quiz
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FOUNDATIONAL CONCEPT 1: BIOMOLECULES AND METABOLISM
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A lab studies the tryptophan (trp) operon in a Gram-negative bacterium. The operon is regulated by a Trp repressor that binds the operator only when complexed with tryptophan, and by attenuation that depends on translation of a leader peptide containing two adjacent Trp codons. Cultures are grown in minimal media with either high or low tryptophan. A mutant strain carries a point mutation that changes one of the leader peptide's Trp codons to a Phe codon (UUC), without altering the downstream structural genes.
Which observation is most consistent with the regulatory mechanism described?
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