Bio & Biochem Quiz
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FOUNDATIONAL CONCEPT 1: BIOMOLECULES AND METABOLISM
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A researcher introduces a single abasic site (a missing base) into a plasmid and replicates it in two yeast strains. Strain 1 has intact high-fidelity replication proteins; Strain 2 lacks a specialized damage-tolerant polymerase. In Strain 2, replication frequently stops at the abasic site and plasmid recovery is low; in Strain 1, plasmid recovery is higher but sequencing shows increased point mutations near the abasic site. Based on the mechanism, which outcome is most consistent with the function of the specialized damage-tolerant polymerase?
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