Bio & Biochem Quiz
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FOUNDATIONAL CONCEPT 1: BIOMOLECULES AND METABOLISM
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A researcher introduces a disulfide bond (Cys substitutions) to "lock" an enzyme in a closed conformation. The oxidized (disulfide formed) enzyme binds substrate tightly but releases product slowly, resulting in reduced overall turnover. Reducing agent restores turnover. Which mechanism best explains the observed enzyme activity?
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