Genetics Quiz
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GENE REGULATION
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The cytotoxic necrotizing factor 1 (CNF1) is a toxin produced by pathogenic E. coli. It functions as an enzyme that targets host cell Rho GTPases (e.g., RhoA, Rac1). CNF1 catalyzes the deamidation of a specific glutamine residue (Gln63 in RhoA) to glutamate within the GTPase's switch II loop. This modification impairs the intrinsic and GAP-stimulated GTP hydrolysis activity of the GTPase. What is the functional consequence of this post-translational modification?
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