Genetics Quiz
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DATA INTERPRETATION & EXPERIMENTAL DESIGN
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In a yeast two-hybrid (Y2H) screen designed to find proteins ("Prey") that interact with a specific protein of interest ("Bait"), the interaction reconstitutes a transcription factor that activates a reporter gene, allowing yeast to grow on selective media. A common artifact is that the Bait protein itself can weakly activate the reporter gene, leading to many false positives. What is the most important control to perform to test for this specific artifact?
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