Cell Biology Quiz
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In a study of lysosomal function, a researcher loads cells with LysoTracker Red (which accumulates in acidic compartments) and observes bright punctate fluorescence throughout the cytoplasm. Treatment with bafilomycin A1 causes a gradual decrease in LysoTracker fluorescence over 2 hours. However, when the researcher simultaneously monitors cathepsin D activity using a fluorogenic substrate, enzyme activity remains high even after LysoTracker signal is lost. What do these observations indicate about lysosomal function under bafilomycin treatment?
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