Cell Biology Quiz
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An immunofluorescence protocol uses mouse anti-BrdU primary antibody to detect DNA synthesis in cells that were pulse-labeled with BrdU. The standard protocol includes DNA denaturation with 2M HCl treatment before antibody incubation. When a researcher omits the HCl treatment step, no BrdU signal is detected, even though the cells were confirmed to incorporate BrdU by an alternative detection method. However, other immunofluorescence antibodies work normally on cells processed without HCl treatment. What is the specific requirement for HCl treatment in BrdU detection?
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