Biochemistry Quiz
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NUCLEOTIDES, DNA/RNA & INFORMATION FLOW
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A graduate student discovers a new bacterial transcriptional regulator protein that binds to a specific DNA sequence upstream of several metabolic genes. When this protein is purified and tested in vitro, it shows strong DNA binding activity at pH 7.0 but no detectable binding at pH 6.0. However, when the same protein is tested in the presence of its target metabolite, it binds DNA strongly at both pH 7.0 and pH 6.0. What is the most likely mechanism for this pH-dependent regulation?
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