Biochemistry Quiz
14 questions · no time limit
0:00
BIOCHEMICAL TECHNIQUES & DATA INTERPRETATION
Question 1 of 14
No time limit
Current
Answered
Flagged
Not seen
PASSAGE
An enzyme assay for lactate dehydrogenase is performed, which catalyzes the reaction: Pyruvate + NADH + H⁺ ⇌ Lactate + NAD⁺. The reaction is monitored using a spectrophotometer set to 340 nm, the wavelength where NADH strongly absorbs light but NAD⁺ does not. The results are plotted as Absorbance at 340 nm (A₃₄₀) versus time. The resulting graph is a straight line with a negative slope. The line starts at an absorbance of 1.0 at time zero and reaches an absorbance of 0.6 at 2 minutes.
What does the slope of this line directly represent?
YOUR PROGRESS
14
QUESTIONS
Ready when you are
Answer in any order. Your progress shows here as you go.
QUESTIONS
KEYBOARD
Pick an answer ABCD
Next question ↵→
Previous ←
Flag for review F
Answers save as you go · flagged questions come back at the end