AP Biology Quiz
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CELLULAR ENERGETICS
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An enzyme is assayed at constant enzyme concentration and saturating substrate concentration. When the temperature is increased from 20°C to 35°C, the initial rate increases. When temperature is further increased to 60°C, the initial rate drops sharply, and repeating the assay at 35°C with the same enzyme sample does not restore the original rate. pH is constant and substrate remains saturating. Which explanation best accounts for the persistent loss of activity after exposure to 60°C?
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