AP Biology Quiz
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GENE EXPRESSION AND REGULATION
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A researcher performs Western blotting to detect a specific membrane protein in two cell types. Proteins from each sample are denatured with SDS, separated by polyacrylamide gel electrophoresis, and transferred to a membrane. The membrane is incubated with a primary antibody specific to the target protein, then with a labeled secondary antibody. A band appears at ~55 kDa in cell type 1 but not in cell type 2. Which conclusion best accounts for the observed banding pattern?
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