Test: GRE Subject Test: Biology

1.

Which of the following accounts for the inability of dideoxynucleotide triphosphates to further polymerize in Sanger DNA sequencing?

Presence of a hydroxyl group on the 2' carbon

Lack of a hydroxyl group on the 2' carbon

Lack of a hydroxyl group on the 3' carbon

Presence of a hydroxyl group on the 3' carbon

Lack of a triphosphate moiety on the 5' carbon

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